Homeopathy
DOI: 10.1055/s-0043-1770360
Clinical Case Series

Homeopathic Sulphur Ointment as Adjuvant in the Treatment of Sheep with Myiasis by Cochliomyia hominivorax (Diptera: Calliphoridae): A Case Series

1   Departamento de Zootecnia e Desenvolvimento Rural, Programa de Pós Graduação em Agroecossistemas, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil
,
Denise Pereira Leme
1   Departamento de Zootecnia e Desenvolvimento Rural, Programa de Pós Graduação em Agroecossistemas, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil
,
Laura Livia Arias Avilés
1   Departamento de Zootecnia e Desenvolvimento Rural, Programa de Pós Graduação em Agroecossistemas, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil
,
Patrizia Ana Bricarello
1   Departamento de Zootecnia e Desenvolvimento Rural, Programa de Pós Graduação em Agroecossistemas, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil
› Author Affiliations
Funding This study was supported by the National Council for Scientific and Technological Development—CNPq (CNPq Grant number: 402867/2017-3) and Milligramm Homeopathic Pharmacy, Florianópolis, SC, Brazil (CNPJ: 15.392.920/0001-70). Barros GP and Avilés LLA received financial support from CNPq and Barros GP received financial support from Coordination of Superior Level Staff Improvement—Brazil (CAPES)—Financing Code 001.

Abstract

Background Myiasis by Cochliomyia hominivorax (Diptera: Calliphoridae) is a serious problem in animal health. Homeopathic Sulphur presents similarity with the clinical presentation verified in these cases and has already shown in vitro efficacy on the blowfly that causes this pathology. This article presents the report of a series of clinical cases of myiasis by C. hominivorax in sheep that underwent adjuvant treatment with homeopathic Sulphur and presents a theoretical-scientific discussion of biological events related to the clinical treatment of the condition.

Patients Seven naturally occurring myiases affecting sheep from an experimental farm were treated by an ointment impregnated with the homeopathic medicine Sulph 12cH, used as adjuvant after the manual removal of accessible larvae from the wound during wound cleaning. The animals were treated daily and evaluated clinically until complete healing of the lesions. Clinical information on the evolution of the inflammatory and scarring process was collected. Blood counts and bacteriological examinations were performed before and after topical homeopathic treatment.

Results The homeopathic ointment Sulph 12cH was able to inhibit the development of parasitic C. hominivorax larvae and promote tissue repair and healing in naturally occurring myiasis in the sheep of this study.

Conclusion The homeopathic medicine Sulphur, formulated as an ointment, may be a useful new and adjuvant therapeutic option for treating myiasis in animals.



Publication History

Received: 04 March 2023

Accepted: 24 April 2023

Article published online:
27 September 2023

© 2023. Faculty of Homeopathy. This article is published by Thieme.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 
  • References

  • 1 Hall M, Wall R. Myiasis of humans and domestic animals. Adv Parasitol 1995; 35: 257-334
  • 2 Costa-Júnior LM, Chaves DP, Brito DRB, Santos VAFD, Costa-Júnior HN, Barros ATM. A review on the occurrence of Cochliomyia hominivorax (Diptera: Calliphoridae) in Brazil. Rev Bras Parasitol Vet 2019; 28: 548-562
  • 3 Pezzi M, Bonacci T, Leis M. et al. Myiasis in domestic cats: a global review. Parasit Vectors 2019; 12: 372
  • 4 Cansi ER, Demo C. Ocorrência de miíases em animais de companhia no Distrito Federal, Brasil. Acta Sci Vet 2011; 39: 982
  • 5 Francesconi F, Lupi O. Myiasis. Tropical Dermatology: 2nd ed. 2016: 393-400
  • 6 Sunny B, Sulthana L, James A, Sivakumar T. Maggot infestation: various treatment modalities. J Am Coll Clin Wound Spec 2018; 8: 51-53
  • 7 Hall MJR, Wall RL, Stevens JR. Traumatic myiasis: a neglected disease in a changing world. Annu Rev Entomol 2016; 61: 159-176
  • 8 de Barros GP, Bricarello PA. Myiasis by Cochliomyia hominivorax (Coquerel, 1858): a neglected zoonosis in Brazil. Open J Vet Med 2020; 10: 80-91
  • 9 Grisi L, Leite RC, Martins JRS. et al. Reassessment of the potential economic impact of cattle parasites in Brazil. Rev Bras Parasitol Vet 2014; 23: 150-156
  • 10 Francesconi F, Lupi O. Myiasis. Clin Microbiol Rev 2012; 25: 79-105
  • 11 Holanda LF, Pereira BJA, De Holanda CVM, De Oliveira JG. Cerebral myiasis. Vol. 84, Neurology. Lippincott Williams and Wilkins; 2015: 434-435
  • 12 Singh S, Athar M, Chaudhary A, Vyas A, Tiwari S, Singh S. Effect of Ivermectin on wound myiasis—a hospital based study. Ann Clin Lab Res 2017; 05: 10-11
  • 13 Ribeiro FAQ, Pereira CSB, Alves A, Marcon MA. Treatment of human cavitary myiasis with oral ivermectin. Rev Bras Otorrinolaringol 2001; 67: 755-761
  • 14 Durighetto Jr AF, Machado MI, Favoreto Jr S, Magalhães AO. Miíases orais: aspectos clínicos e laboratoriais de um caso humano. Rev Odontol Bras Central. 1995; 5: 19-22
  • 15 Singh A, Singh Z. Incidence of myiasis among humans – a review. Parasitol Res 2015; 114: 3183-3199
  • 16 da Silva BB, Borges US, Pimentel ICC. Human vaginal myiasis caused by Cochliomyia hominivorax . Int J Gynaecol Obstet 2005; 89: 152-153
  • 17 Shinohara EH, Martini MZ, de Oliveira Neto HG, Takahashi A. Oral myiasis treated with ivermectin: case report. Braz Dent J 2004; 15: 79-81
  • 18 Antunes AA, Santos TS, Avelar RL, Martins Neto EC, Macedo Neres B, Laureano Filho JR. Oral and maxillofacial myiasis: a case series and literature review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112: e81-e85
  • 19 Osorio J, Moncada L, Molano A, Valderrama S, Gualtero S, Franco-Paredes C. Role of ivermectin in the treatment of severe orbital myiasis due to Cochliomyia hominivorax . Clin Infect Dis 2006; 43: e57-e59
  • 20 Campbell WC. Lessons from the history of ivermectin and other antiparasitic agents. Annu Rev Anim Biosci 2016; 4: 1-14
  • 21 Omura S, Crump A. Ivermectin: panacea for resource-poor communities?. Trends Parasitol 2014; 30: 445-455
  • 22 Barros ATM, Ravaglia E, Aquino W da S, Passos WM, Leite LTB. Eficácia de endectocidas em infestações naturais por Cochliomyia hominivorax (Mosca-varejeira) em bezerros no Pantanal. Embrapa Pantanal-Circular Técnica 2010. 90. 1-4
  • 23 Lopes WDZ, Teixeira WFP, Felippelli G. et al. Ivermectina e abamectina em diferentes doses e vias de aplicação contra larvas de Cochliomyia hominivorax em bolsas escrotais de bovinos recém-castrados, provenientes da região sudeste do Brasil. Cienc Rural 2013; 43: 2195-2201
  • 24 de Carvalho RA. Bases moleculares da resistência a inseticidas organofosforados em Cochliomyia hominivorax (Diptera: Calliphoridae). Thesis (Doctorate)—Instituto de Biologia. Universidade Estadual de Campinas. Campinas; 2010
  • 25 Lumaret JP, Errouissi F, Floate K, Römbke J, Wardhaugh K. A review on the toxicity and non-target effects of macrocyclic lactones in terrestrial and aquatic environments. Curr Pharm Biotechnol 2012; 13: 1004-1060
  • 26 Leu AF. Pesticide safety in livestock products. Managing Healthy Livestock Production and Consumption. Academic Press; 2022: 73-91
  • 27 Pavela R. Essential oils for the development of eco-friendly mosquito larvicides: A review. Ind Crops Prod 2015; 76: 174-187
  • 28 Isman MB. A renaissance for botanical insecticides?. Pest Manag Sci 2015; 71: 1587-1590
  • 29 Aleixo DL, Bonamin LV, Ferraz FN, Da Veiga FK, De Araújo SM. Homeopathy in parasitic diseases. Int J High Dilution Res 2014; 13: 13-27
  • 30 Boericke W. Matéria médica homeopática com índice terapêutico. 2nd ed. Fraenkel Vervolet, ed. Rio de Janeiro; 1993: 561
  • 31 Percie du Sert N, Ahluwalia A, Alam S. et al. Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0. PLoS Biol 2020; 18: e3000411
  • 32 Moya-Borja GE, Oliveira CMB, Muniz RA, Goncalves LCB. Prophylactic and persistent efficacy of doramectin against Cochliomyia hominivorax in cattle. Vet Parasitol 1993; 49: 95-105
  • 33 Moya-Borja GE, Muniz RA, Umehara O, Goncalves LCB, Silva DSF, McKenzie ME. Protective efficacy of doramectin and ivermectin against Cochliomyia hominivorax . Vet Parasitol 1997; 72: 101-109
  • 34 Correia TR, Scott FB, Verocai GG. et al. Larvicidal efficacy of nitenpyram on the treatment of myiasis caused by Cochliomyia hominivorax (Diptera: Calliphoridae) in dogs. Vet Parasitol 2010; 173: 169-172
  • 35 Oliveira PC, Moraes PA, Scott FB, Verocai GG, Correia TR, Fernandes JI. Efficacy of spinosad on the treatment of myiasis caused by Cochliomyia hominivorax (Diptera: Calliphoridae) in dogs. Vet Parasitol 2018; 258: 53-56
  • 36 Brasil.. Agencia Nacional de Vigilância Sanitária—ANVISA. Farmacopeia Brasileira. RDC N° 17 de. 2010 : Dispõe sobre as Boas Práticas de Fabricação de Medicamentos.
  • 37 Brasil.. Agencia Nacional de Vigilância Sanitária—ANVISA Farma-copeia Homeopática Brasileira, 3rd ed. São Paulo. 2011
  • 38 Barros GP, Avilés LLA, Hillmann MCH, Bricarello PA. Effect of ointments of Sulphur or Pyrogenium on larvae of Cochliomyia hominivorax (Diptera: Calliphoridae). Homeopathy 2022; 111: 271-277
  • 39 Oliveira BGR, Castro JBDA, Andrade NC. Técnicas Utilizadas na Aferição de Feridas e Avaliação do Processo Cicatricial. Saúde Coletiva. 2005; 2: 57-62
  • 40 Prata M. Uso tópico do açúcar em ferida cutânea: estudo experimental em rato. Acta Cir Bras 1988; 3: 43-48
  • 41 Ramsey DT, Pope ER, Wagner-Mann C, Berg JN, Swaim SF. Effects of three occlusive dressing materials on healing of full-thickness skin wounds in dogs. Am J Vet Res 1995; 56: 941-949
  • 42 Thrall MA, Baker DC, Campbell TW. et al. Hematologia e Bioquímica Clínica Veterinária.1st ed. São Paulo: Roca Editora; 2007: 582
  • 43 Carter GR. Fundamentos de Bacteriologia e Micologia Veterinária. São Paulo: Roca Editora; 1988: 118-121
  • 44 Pugh DG, Baird AN. Sheep and Goat Medicine. Sheep and Goat Medicine. 2nd ed. Elsevier Health Sciences; 2012: 640
  • 45 Gauthier C, Griffin G. Using animals in research, testing and teaching. Rev Sci Tech 2005; 24: 735-745
  • 46 Sanavria A, Prata MCA. Eficácia profilática do doramectin contra infestações naturais por Cochliomyia hominivorax em ovinos pós-castração. Rev Bras Parasitol Vet 1996; 5: 113-117
  • 47 Sanavria A, Muniz RA, Gonçalves LCB, Rew RS, Silva DS. Eficácia profilática de doramectin contra infestações naturais por Cochliomyia hominivorax em bovinos após castração. Rev Bras Parasitol Vet 1996; 5: 7-10
  • 48 Brisola C, Jacqueline P. Myiasis-causing flies. In: Encyclopedia of Infection and Immunity. Elsevier Inc.; 2020: 1-11
  • 49 Oliveira CMB. Biologia, Flutuação Populacional e Patologia da Cochliomyia hominivorax (Coquerel, 1858) (Diptera: Calliphoridae). Thesis (Doctorate). Universidade Federal Rural do Rio de Janeiro; 1980
  • 50 Amos Cde A. Atividade terapêutica do spinosad contra larvas de Cochliomyia hominivorax (L1, L2 e L3) em bovinos infestados natural e artificialmente [Masters dissertation]. Universidade Estadual de São Paulo; 2009
  • 51 Coelho HE. Patologia veterinária. 1st ed. Rio de Janeiro : Manole Editora ; 2002: 235
  • 52 Balsa IM, Culp WTN. Wound care. Vet Clin North Am Small Anim Pract 2015; 45: 1049-1065
  • 53 Woodlands C. Wound management in veterinary practice. Vet Nurs 2014; 29: 83-86
  • 54 Passeti TA, Bissoli LR, Macedo AP, Libame RB, Diniz S, Waisse S. Action of antibiotic oxacillin on in vitro growth of methicillin-resistant Staphylococcus aureus (MRSA) previously treated with homeopathic medicines. Homeopathy 2017; 106: 27-31
  • 55 Nayak C, Sahoo AR, Nayak C. Thlaspi bursa pastoris in treatment of urinary stones: a case series. Homeopathy 2020; 109: 238-242
  • 56 Chand KS, Kapoor P. Two case reports of integrated management of antibiotic-resistant urinary tract infection. Homeopathy 2020; 109: 97-106
  • 57 Spellberg B, Guidos R, Gilbert D. et al; Infectious Diseases Society of America. The epidemic of antibiotic-resistant infections: a call to action for the medical community from the Infectious Diseases Society of America. Clin Infect Dis 2008; 46: 155-164
  • 58 Anderson RM. The pandemic of antibiotic resistance. Nat Med 1999; 5: 147-149