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Synthesis 2009(7): 1224-1226  
DOI: 10.1055/s-0028-1088010
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© Georg Thieme Verlag Stuttgart ˙ New York

Synthesis of Regioisomerically Pure 5-Functionalized 2′,7′-Dichloro­fluoresceins

Juan C. Castro, Andrey Malakhov, Kevin Burgess*
Department of Chemistry, Texas A & M University, P.O. Box 30012, College Station, TX 77842, USA
Fax: +1(979)8458839; e-Mail: burgess@tamu.edu;
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Publication History

Received 12 November 2008
Publication Date:
06 March 2009 (online)

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Abstract

Regioisomerically pure 2′,7′-dichloro-5-nitrofluorescein was isolated on a multigram scale, and used as an intermediate for the generation of three compounds that might be used for labeling biomolecules, namely the corresponding amino, azido, and (4,6-dichloro-1,3,5-triazin-2-ylamino) derivatives.

Key words

fluorescence - chromophores - condensation - heterocycles - regioselectivity

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