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Z Geburtshilfe Neonatol 2005; 209(2): 59-64
DOI: 10.1055/s-2005-864115
Originalarbeit
© Georg Thieme Verlag KG Stuttgart · New York

Stimulierung der hCG-, Estrogen- und Progesteronproduktion in isolierten Trophoblastzellen durch Glycodelin A bzw. seine N-Glycane

Stimulation of hCG, Estrogen and Progesterone Production in Isolated Trophoblast Cells by Glycodelin A or its N-GlycansU. Jeschke1 , D.-U. Richter2 , V. Briese2 , I. Mylonas1 , K. Friese1
  • 1Ludwig Maximilians Universität München, I. Frauenklinik-Innenstadt (Klinikdirektor: Prof. Dr. med. habil. K. Friese), Maistrasse 11, 80337 München
  • 2Frauenklinik und Poliklinik der Universität Rostock, Doberaner Str. 142, 18055 Rostock
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Publication History

Eingereicht: 11.11.2004

Angenommen nach Überarbeitung: 8.2.2005

Publication Date:
26 April 2005 (online)

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Zusammenfassung

Fragestellung: Das immunsuppressive Plazentaprotein Glycodelin A (GdA) wird in hohen Konzentrationen im ersten Trimester der Schwangerschaft vom sekretorischen Endometrium und der Decidua sezerniert. Der Anteil von GdA an der durch die Decidua freigesetzten Proteinmenge beträgt in dieser Zeit 10 %. GdA besitzt ungewöhliche Kohlenhydratstrukturen, zu denen insbesondere fukosylierte LacdiNAc-Strukturen gehören. In der vorliegenden Studie beschreiben wir die Wirkung von GdA bzw. seiner N-Glycane auf die hCG-, Estogen- und Progesteron-Freisetzung von Trophoblastzellen in vitro. Material und Methodik: Die Isolierung von Trophoblastzellen erfolgte aus zum Termin geborener Plazenta durch Fragmentierung einzelner Kotyledonen und anschließendem Verdau mit Hilfe von Trypsin/DNAse. Trophoblastzellen wurden mit unterschiedlichen GdA-Glycanen inkubiert. Die Produktion von hCG, Estrogen und Progesteron wurde nach 24 und 48 Stunden gemessen. Ergebnisse: Mit GdA bzw. GdA-Glycanen versetzte Trophoblastzellen weisen in vitro eine erhöhte hCG-, Estrogen- und Progesteron-Freisetzung im Vergleich zu unbehandelten Zellen auf. Schlussfolgerung: Progesteron, Estrogen und hCG sind „Marker” für den Differenzierungsprozess von Trophoblastzellen. Das mütterliche Protein Glycodelin A und insbesondere seine Kohlenhydratmoleküle scheinen fetale Trophoblastzellen in der Differenzierung zu stimulieren.

Abstract

The immunosuppressive protein glycodelin A (GdA) is secreted by decidual tissue in high rates in the first trimester of pregnancy. GdA forms about 10 % of the total protein amount released by the decidua in the first trimester of pregnancy and has a unique glycosylation including fucosylated LacdiNAc structures. The purpose of this study was to investigate the effect of glycodelin A and its N-glycans on hCG, estrogen and progesterone release by cytotrophoblasts in vitro. Trophoblast cells were isolated from human term placenta by fragmentation of villous tissue. Trophoblast cells fuse in vitro to syncytiotrophoblast cells. Trophoblast cells were incubated with GdA and various glycans of GdA. The production of hCG and progesterone was measured after 24 and 48 hours. The production of hCG, estrogen and progesterone was increased in GdA and glycan-treated cell cultures as compared to untreated trophoblast cell cultures. Conclusion: HCG, estrogen and progesterone are markers for the differentiation process of cytotrophoblast cells. The results suggest that GdA with its distinct glycosylation modulates the differentation of trophoblasts.

Schlüsselwörter

Glycodelin A - hCG - Progesteron - Trophoblastzellen - Kohlenhydratstrukturen

Key words

Glycodelin A - hCG - estrogen - progesterone - trophoblast cells - carbohydrates

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PD Dr. Udo Jeschke

Ludwig Maximilians Universität München

I. Frauenklinik-Innenstadt

Maistrasse 11

80337 München

Phone: 089 5160 4266

Fax: 089 5160 4916

Email: udo.jeschke@med.uni-muenchen.de