Benzimidazole-based nucleotides and dinucleotides have been synthesised to increase
the range of chemical tools available to probe the NAD+ biology space. They were examined for their reactivity in alkylation-type reactions,
where they yielded unstable alkylated heteoaromatic adducts, both chemically and enzymatically.
While unsuited for NAD+ cyclases, these NAD+ analogues could be viable substrates for non-adenine modifying NAD+-dependent enzyme classes.
Key words
nucleotides - benzimidazoribosyl nucleosides - phosphorylation - cyclic adenosine
diphosphoribose - enzymes