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Synlett 2011(11): 1486-1494
DOI: 10.1055/s-0030-1260583
DOI: 10.1055/s-0030-1260583
ACCOUNT
© Georg Thieme Verlag
Stuttgart ˙ New YorkArtificial Ribozyme-Based Regulators of Gene Expression
Weitere Informationen
Received
23 March 2011
Publikationsdatum:
26. Mai 2011 (online)
Publikationsverlauf
Publikationsdatum:
26. Mai 2011 (online)
Abstract
The development of RNA-based switches of gene expression is summarized. Most switches are based on the Schistosoma mansonii hammerhead ribozyme (HHR), a self-cleaving RNA sequence that is inserted into an mRNA. Control of HHR self-cleavage is achieved by the attachment of an aptamer domain to the HHR scaffold. External addition of a small-molecule ligand regulates catalytic activity of the ribozyme and hence gene expression. These so-called aptazymes are suited to control several classes of RNA. In addition to mRNA, we have incorporated artificial RNA switches into tRNA as well as the bacterial 16S rRNA. In addition, the presented switches should be widely applicable as demonstrated by experiments in bacteria, yeast, and mammals.
1 Introduction
2 Ribozymes in Nature
3 Ribozymes as Parts for Artificial mRNA Switches
4 Regulation of Further RNA Classes
5 Conclusion
Key words
ribonucleic acid - aptamer - riboswitch - hammerhead - RNA-ligand interaction
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