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DOI: 10.1055/a-2685-9396
Pseudouridine-Modified Phosphorodiamidate Morpholino Oligonucleotides: Synthesis and Biophysical Insights
SS thanks the Department of Science and Technology (DST), New Delhi, Government of India (DST/TDT/TC/RARE/2022/10c2) for grant support.
Abstract
The synthesis of morpholino pseudouridine chlorophosphoramidate monomer from pseudouridine and its incorporation in a 12-mer phosphorodiamidate morpholino oligonucleotide (PMO) has been successfully achieved. Thermal melting (T m) of this PMO was performed with the complementary strand of DNA and RNA. In the case of RNA, the T m of the base pairing between pseudouridine with A and G was comparable. Circular dichroism analysis reveals the B-type helical conformation of PMO–RNA duplexes. These advancements are expected to broaden the potential applications of PMOs in biomedical research.
Keywords
Morpholino pseudouridine - Oligonucleotides - Thermal denaturation analysis - Circular dichroism - Antisense agentsPublikationsverlauf
Eingereicht: 31. Juli 2025
Angenommen nach Revision: 18. August 2025
Accepted Manuscript online:
19. August 2025
Artikel online veröffentlicht:
18. September 2025
© 2025. Thieme. All rights reserved.
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