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Thromb Haemost 2009; 102(06): 1117-1134
DOI: 10.1160/TH09-07-0472
Theme Issue Article
Schattauer GmbH

The contribution of systems biology and reverse genetics to the understanding of Kaposi’s sarcoma-associated herpesvirus pathogenesis in endothelial cells

Michael Stürzl
1   Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany
,
Andreas Konrad
1   Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany
,
Khaled R. Alkharsah
2   Department of Virology, Medical School Hannover, Hannover, Germany
,
Ramona Jochmann
1   Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany
,
Mathias Thurau
1   Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany
,
Gaby Marquardt
1   Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nuremberg, Erlangen, Germany
,
Thomas F. Schulz
2   Department of Virology, Medical School Hannover, Hannover, Germany
› Author AffiliationsFinancial support: This work was supported by grants of the Deutsche Forschungsgemeinschaft (DFG-SPP 1130 to MS and TFS; DFG-SFB 566 to TFS; DFG-GK 1071 and DFG 317/2-1 to MS), the Interdisciplinary Center for Clinical Research (IZKF) of the University of Erlangen-Nuremberg (to M.S) and the European Union (FP6 Integrated project INCALSHC- CT-2005-018704 to TFS).
Further Information

Publication History

Received: 22 July 2009

Accepted after minor revision: 05 November 2009

Publication Date:
28 November 2017 (online)

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Summary

Kaposi’s sarcoma-associated herpesvirus (KSHV) / human herpesvirus-8 is the causative agent of the endothelial cell-derived tumour Kaposi’s sarcoma. Herpesviruses possess large complex genomes which provide many options to regulate cellular physiology during the viral life cycle and in the course of tumourigenicity. Novel techniques of systems biology and reverse genetics are increasingly applied to dissect the complex interaction of KSHV with endothelial cells. This review will outline novel results and pitfalls of these technologies in the elucidation of KSHV pathogenicity.

Keywords

Endothelial cells - angiogenesis - apoptosis - NF-kappaB - interferon
 

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