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J Reconstr Microsurg 2002; 18(2): 115-120
DOI: 10.1055/s-2002-19892
Copyright © 2002 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA. Tel.: +1(212) 584-4662

Effect of Hyperthermic Preconditioning on the Survival of Ischemia-Reperfused Skin Flaps: A New Skin-Flap Model in the Mouse

Trinh Cao Minh1 , Shigeru Ichioka2 , Takashi Nakatsuka2 , Junsuke Kawai1 , Masahiro Shibata3 , Joji Ando3 , Kiyonori Harii1
  • 1Department of Plastic and Reconstructive Surgery, University of Tokyo, Tokyo, Japan
  • 2Department of Plastic and Reconstructive Surgery, Saitama Medical School, Saitama, Japan
  • 3Department of Biomedical Engineering, University of Tokyo, Japan
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Publication History

Publication Date:
31 January 2002 (online)

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ABSTRACT

To investigate whether hyperthermic preconditioning can actually protect skin flaps against ischemia/reperfusion injury, the authors first developed a new skin-flap model in 15 mice, a dorsal bipedicle island skin-flap model. Then, another 75 mice were separated into five groups. Mice in Groups 1 to 4 received the same hyperthermic preconditioning, but had different recovery times of 6 hr, 24 hr, 48 hr, and 72 hr, respectively. Mice in Group 5 served as control. Island skin flaps were elevated in all groups, and then were subjected to 8 hr of ischemia and subsequent reperfusion. Flap survival was statistically significantly higher than in controls in animals in Groups 1 and 3, with recovery times of 6 hr and 48 hr, respectively. Mice in Groups 2 and 4 had recovery times of 24 hr and 72 hr, respectively. Hyperthermic preconditioning could thus protect skin flaps against ischemia/reperfusion injury, and there were two optimal periods for such a protective effect.

KEYWORD

Mouse - skin flap - hyperthermic preconditioning - heat shock

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