J Reconstr Microsurg 2016; 32(09): 699-705
DOI: 10.1055/s-0036-1586749
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

A Novel Ex Vivo Training Model for Acquiring Supermicrosurgical Skills Using a Chicken Leg

Ignacio J. Cifuentes
1   Section of Plastic and Reconstructive Surgery, School of Medicine, Pontificia Universidad Católica de Chile, Chile
,
José R. Rodriguez
1   Section of Plastic and Reconstructive Surgery, School of Medicine, Pontificia Universidad Católica de Chile, Chile
,
Ricardo A. Yañez
1   Section of Plastic and Reconstructive Surgery, School of Medicine, Pontificia Universidad Católica de Chile, Chile
,
María C. Salisbury
1   Section of Plastic and Reconstructive Surgery, School of Medicine, Pontificia Universidad Católica de Chile, Chile
,
Álvaro J. Cuadra
1   Section of Plastic and Reconstructive Surgery, School of Medicine, Pontificia Universidad Católica de Chile, Chile
,
Julian E. Varas
2   Experimental Surgery and Simulation Center, School of Medicine, Pontificia Universidad Católica de Chile, Chile
,
Bruno L. Dagnino
1   Section of Plastic and Reconstructive Surgery, School of Medicine, Pontificia Universidad Católica de Chile, Chile
› Author Affiliations
Further Information

Publication History

29 December 2015

22 June 2016

Publication Date:
19 August 2016 (online)

Abstract

Background Supermicrosurgery is a technique used for dissection and anastomosis of submillimeter diameter vessels. This technique requires precise hand movements and superb eye–hand coordination, making continuous training necessary. Biological in vivo and ex vivo models have been described for this purpose, the latter being more accessible and cost-effective. The aim of this study is to present a new ex vivo training model using a chicken leg.

Methods In 28 chicken legs, an anatomical study was performed. An intramuscular perforator vessel was identified and dissected. Arterial diameters of 0.7, 0.5, and 0.3 mm were identified and consistency of the perforator was assessed. In additional 10 chicken legs, 25 submillimeter arteries were anastomosed using this perforator vessel. Five arteries of 0.3 and 10 of 0.5 mm were anastomosed with nylon 11–0 and 12–0 sutures. Intravascular stent (IVaS) technique and open guide (OG) technique were used in 0.5-mm arteries. A total of 10 arteries of 0.7 mm were anastomosed using 10–0 sutures in a conventional fashion. Dissection and anastomosis time were recorded and patency was tested.

Results We were able to identify 0.7 to 0.3 mm diameter arteries in all the specimens and confirm the consistency of the perforator. The median time for dissection was 13.4 minutes. The median time for anastomosis was 32.3 minutes for 0.3-mm arteries, 24.3 minutes for 0.5-mm arteries using IVaS, 29.5 minutes for the OG technique, and 20.9 minutes for the 0.7 mm diameter arteries. All the anastomoses were permeable.

Conclusion Due to its consistent and adequate diameter vessels, this model is adequate for training supermicrosurgical skills.

Note

The corresponding author receives financial support from a local medical supply distributor, Medcorp SA, which has no involvement in the present study.


 
  • References

  • 1 Koshima I, Yamamoto T, Narushima M, Mihara M, Iida T. Perforator flaps and supermicrosurgery. Clin Plast Surg 2010; 37 (4) 683-689 , vii–iii
  • 2 Masia J, Olivares L, Koshima I , et al. Barcelona consensus on supermicrosurgery. J Reconstr Microsurg 2014; 30 (1) 53-58
  • 3 Koshima I. Microsurgery in the future: Introduction to supra-microsurgery and perforator flaps. Special invited lecture at the 1st International Course on Perforator Flap and Arterialized Skin Flaps; 1997; Gent, Belgium
  • 4 Hong JP. The use of supermicrosurgery in lower extremity reconstruction: the next step in evolution. Plast Reconstr Surg 2009; 123 (1) 230-235
  • 5 Foo TL. Open guide suture technique for distal fingertip replantation. J Plast Reconstr Aesthet Surg 2013; 66 (3) 443-444
  • 6 Yamamoto T, Koshima I. Supermicrosurgical anastomosis of superficial lymphatic vessel to deep lymphatic vessel for a patient with cellulitis-induced chronic localized leg lymphedema. Microsurgery 2015; 35 (1) 68-71
  • 7 Narushima M, Koshima I, Mihara M, Uchida G, Gonda K. Intravascular stenting (IVaS) for safe and precise supermicrosurgery. Ann Plast Surg 2008; 60 (1) 41-44
  • 8 Wei FC, Mancer K, Zuker RM. The temporary stent technique: an easier method of micro-venous anastomosis. Br J Plast Surg 1982; 35 (1) 92-95
  • 9 Özkan O, Ozgentaş HE. Open guide suture technique for safe microvascular anastomosis. Ann Plast Surg 2005; 55 (3) 289-291
  • 10 Miyamoto S, Sakuraba M, Asano T , et al. Optimal technique for microvascular anastomosis of very small vessels: Comparative study of three techniques in a rat superficial inferior epigastric arterial flap model. J Plast Reconstr Aesthet Surg 2010; 63 (7) 1196-1201
  • 11 Liu HL. Microvascular anastomosis of submillimeter vessels-a training model in rats. J Hand Microsurg 2013; 5 (1) 14-17
  • 12 Mofikoya BO, Ugburo AO, Bankole OB. Microvascular anastomosis of vessels less than 0.5 mm in diameter: a supermicrosurgery training model in Lagos, Nigeria. J Hand Microsurg 2011; 3 (1) 15-17
  • 13 Yamashita S, Sugiyama N, Hasegawa K, Namba Y, Kimata Y. A novel model for supermicrosurgery training: the superficial inferior epigastric artery flap in rats. J Reconstr Microsurg 2008; 24 (8) 537-543
  • 14 Steffens K, Koob E, Hong G. Training in basic microsurgical techniques without experiments involving animals. Arch Orthop Trauma Surg 1992; 111 (4) 198-203
  • 15 Chen WF, Eid A, Yamamoto T, Keith J, Nimmons GL, Lawrence WT. A novel supermicrosurgery training model: the chicken thigh. J Plast Reconstr Aesthet Surg 2014; 67 (7) 973-978
  • 16 Levinsohn EM, Packard Jr DS, West EM, Hootnick DR. Arterial anatomy of chicken embryo and hatchling. Am J Anat 1984; 169 (4) 377-405
  • 17 Matsumura N, Horie Y, Shibata T, Kubo M, Hayashi N, Endo S. Basic training model for supermicrosurgery: a novel practice card model. J Reconstr Microsurg 2011; 27 (6) 377-382
  • 18 Dawe SR, Pena GN, Windsor JA , et al. Systematic review of skills transfer after surgical simulation-based training. Br J Surg 2014; 101 (9) 1063-1076
  • 19 Couceiro J, Ozyurekoglu T, Sanders S, Tien H. Microsurgical training regimen with nonliving chicken models. Microsurgery 2013; 33 (3) 251-252
  • 20 Hino A. Training in microvascular surgery using a chicken wing artery. Neurosurgery 2003; 52 (6) 1495-1497 , discussion 1497–1498
  • 21 Bates BJ, Wimalawansa SM, Monson B, Rymer MC, Shapiro R, Johnson RM. A simple cost-effective method of microsurgical simulation training: the turkey wing model. J Reconstr Microsurg 2013; 29 (9) 615-618
  • 22 Nam SM, Shin HS, Kim YB, Park ES, Choi CY. Microsurgical training with porcine thigh infusion model. J Reconstr Microsurg 2013; 29 (5) 303-306
  • 23 Onoda S, Kimata Y, Sugiyama N , et al. Analysis of 10-year training results of medical students using the microvascular research center training program. J Reconstr Microsurg 2016; 32 (5) 336-341
  • 24 Assersen K, Sørensen J. Intravascular stenting in microvascular anastomoses. J Reconstr Microsurg 2015; 31 (2) 113-118
  • 25 Mihara M, Hayashi Y, Iida T, Narushima M, Koshima I. Instruments for supermicrosurgery in Japan. Plast Reconstr Surg 2012; 129 (2) 404e-406e