The Effect of Basketball Training on the Players' Erythrocyte Membrane Acetylcholinesterase, (Na⁺,K⁺)-ATPase and Mg²⁺-ATPase Activities

Abstract

The aim of this study was to investigate whether the activities of erythrocyte membrane acetylcholinesterase (AChE), (Na⁺,K⁺)-ATPase and Mg²⁺-ATPase are modulated by a basketball training. Blood was obtained from 10 basketball players pre- and postexercise. Total antioxidant status (TAS), lactate and pyruvate concentrations were determined with kits, while the enzyme activities were determined spectrophotometrically. Post-training blood lactate and pyruvate concentrations as well as AChE (2.90 ± 0.05 vs. 3.98 ± 0.09 ΔOD/min · mg protein, p < 0.01) and Na⁺,K⁺-ATPase (0.58 ± 0.04 vs. 1.27 ± 0.12 µmol Pi/h · mg protein, p < 0.001) activities were remarkably increased, whereas TAS was significantly decreased. Mg²⁺-ATPase activity remained unaltered at the end of the training. In conclusion, the stimulation of AChE and Na⁺,K⁺-ATPase by the training may be due to the rise of blood catecholamine oxidation contributing to TAS decrease and/or the increase of serotonin levels. This stress condition may modulate cholinergic and catecholaminergic/serotoninergic functions in players.

Key words

exercise - oxidative stress - ATPase - acetylcholinesterase - erythrocyte membranes - lactate

References

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