Sarpogrelate Hydrochloride, an Antagonist of 5-Hydroxytryptamine Receptor, Improves Skin Flap Survival and Suppresses Thrombus Formation in Injured Microvessels of Rabbits

Maki Toda Takase; Ichiro Hashimoto; Hideki Nakanishi; Yutaka Nakaya; Hiroshi Harada; Hiromichi Sedo; Ikuno Kanda

doi:10.1055/s-2006-931909

Journal of Reconstructive Microsurgery, Table of Contents

J Reconstr Microsurg 2006; 22(1): 059-066
DOI: 10.1055/s-2006-931909

Sarpogrelate Hydrochloride, an Antagonist of 5-Hydroxytryptamine Receptor, Improves Skin Flap Survival and Suppresses Thrombus Formation in Injured Microvessels of Rabbits

Maki Toda Takase¹ , Ichiro Hashimoto¹ , Hideki Nakanishi¹ , Yutaka Nakaya² , Hiroshi Harada³ , Hiromichi Sedo¹ , Ikuno Kanda¹

¹Department of Plastic and Aesthetic Surgery, School of Medicine, University of Tokushima, Tokushima, Japan
²Department of Nutrition, School of Medicine, University of Tokushima, Tokushima, Japan
³Department of Plastic and Reconstructive Surgery, Tokushima Red Cross Hospital, Tokushima, Japan

Abstract

ABSTRACT

The authors carried out two studies to examine the effects of the 5-hydroxytryptamine receptor antagonist, sarpogrelate hydrochloride (SH), on flap necrosis. The first study measured survival rates and included histologic examination of random-pattern skin flaps in rabbits. The second study assessed the time to complete obstruction of blood flow with the artificial thrombus formation method, and the time for leukocytes to adhere to the endothelium of microvessels in a rabbit-ear chamber. The treatment groups were injected with SH. The survival rates of skin flaps in the SH-treated group were increased significantly. Histologic examination of vessels in the control group revealed that vessels in the deep dermis were obstructed completely by thrombi, whereas such vessels were not obstructed in the SH-treated group. Thrombus formation time and leukocyte adhesion time in the SH-treated group were prolonged and decreased, respectively. Thus, SH maintained flap circulation and promoted flap survival by preventing thrombus formation.

KEYWORDS

5-hydroxytryptamine receptor antagonist - flap necrosis - rabbit ear chamber - thrombus formation

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References

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Maki Toda TakaseM.D.

Department of Plastic and Aesthetic Surgery, School of Medicine, University of Tokushima, Kuramoto-2, Tokushima 770-8503, Japan