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Am J Perinatol 2019; 36(11): 1179-1187
DOI: 10.1055/s-0038-1676491
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Obestatin Reduces Intestinal Damage in Experimental Necrotizing Enterocolitis in Newborn Rats

Sabriye Korkut
1   Department of Neonatology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
Ahmet Özdemir
1   Department of Neonatology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
Arzu Hanım Yay
2   Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
Betül Yalçın
2   Department of Histology and Embryology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
Mahir Ceylan
1   Department of Neonatology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
Levent Korkmaz
1   Department of Neonatology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
Cevat Yazıcı
3   Department of Biochemistry, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
İnayet Güntürk
3   Department of Biochemistry, Faculty of Medicine, Erciyes University, Kayseri, Turkey
,
Selim Kurtoğlu
1   Department of Neonatology, Faculty of Medicine, Erciyes University, Kayseri, Turkey
› Author Affiliations
Funding This work was supported by the Scientific Research Projects Unit of Erciyes University (Grant number: TSA-2015–5676).
Further Information

Publication History

14 August 2018

30 October 2018

Publication Date:
19 December 2018 (online)

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Abstract

Objective To investigate the effect of pretreatment with obestatin (OB), an endogenous hormone also found in mother's milk, in experimental necrotizing enterocolitis (NEC).

Study Design Pups were randomized into four groups: control, OB-control, NEC, and OB-NEC. NEC was induced by asphyxia and hypothermia in the NEC and OB-NEC groups. OB was administered to the OB-control and OB-NEC groups. Macroscopic scoring of the intestinal tract was evaluated and tissue samples were obtained for histopathological and biochemical examination on the fourth day.

Results OB improved the macroscopic appearance of the gut and the clinical score during the experiment (p  <  0.05). The rate of occurrence of NEC in the OB-NEC group was lower than the NEC group (p  =  0.001). OB prevented necrosis and reduced the number of apoptotic cells in the OB-NEC group compared with the NEC group (p  =  0.006). Furthermore, interleukin-6 and malondialdehyde levels in the OB-NEC group were lower than the NEC group (p  <  0.05).

Conclusion OB reduced intestinal damage and prevented necrosis through anti-inflammatory and antiapoptotic effects in experimental NEC. This effect of OB should be confirmed in clinical studies. Furthermore, future research should investigate whether OB plays a role in NEC pathogenesis or NEC is associated with OB levels in the serum and in breast milk.

Keywords

necrotizing enterocolitis - obestatin - treatment - rat - newborn