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Am J Perinatol 2015; 32(04): 337-342
DOI: 10.1055/s-0034-1384640
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Rapid Fecal Calprotectin (FC) Analysis: Point of Care Testing for Diagnosing Early Necrotizing Enterocolitis

Alona Bin-Nun
1   Department of Neonatology, Shaare Zedek Medical Center, Jerusalem, Israel
,
Christina Booms
2   Department of Neonatology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
,
Naama Sabag
3   Biochemistry Laboratory, Shaare Zedek Medical Center, Jerusalem, Israel
,
Refael Mevorach
3   Biochemistry Laboratory, Shaare Zedek Medical Center, Jerusalem, Israel
,
Nurit Algur
3   Biochemistry Laboratory, Shaare Zedek Medical Center, Jerusalem, Israel
,
Cathy Hammerman
1   Department of Neonatology, Shaare Zedek Medical Center, Jerusalem, Israel
4   Faculty of Medicine, Hebrew University, Jerusalem, Israel
› Author Affiliations
Further Information

Publication History

23 March 2014

05 June 2014

Publication Date:
11 August 2014 (online)

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Abstract

Objective The aim of this study is to compare fecal calprotectin (FC) levels as measured by a rapid FC assay with those measured by enzyme-linked immunosorbent assay (ELISA) from concurrent stool samples. We also attempted to demonstrate a correlation between elevated rapid assay FC levels and the presence of necrotizing enterocolitis (NEC) and to define a cutoff FC value which could serve as a basis for diagnosing NEC in the future.

Study Design Stool samples were collected for FC analysis at 1 and 3 weeks postnatally and whenever there was clinical suspicion of NEC.

Results Rapid assay FC levels were elevated with NEC (3,000 µg/g stool [2075,7875] vs. without (195 µg/g stool [110,440] p < 0.001); and were well correlated with ELISA FC levels (r 2 = 0.89).

Conclusion We present the first data showing that rapid assay FC levels are potentially useful in the bedside diagnosis of NEC.

Keywords

necrotizing enterocolitis - fecal calprotectin - rapid assay - calprotectin - premature neonates
 

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