Abstract
Background Necrotizing enterocolitis (NEC) and spontaneous intestinal perforation (SIP) are
causes of bowel perforation in premature neonates. Studies have demonstrated that
both are associated with acute chorioamnionitis (ACA) of the placenta.
Aim The aim of our study was to identify any histopathological links between placental
histopathological abnormalities and the later development of NEC and/or SIP in premature
patients presenting at our institution.
Patients and Methods Cases with a diagnosis of NEC/SIP were identified. Entry criteria were the diagnosis
of NEC/SIP was confirmed clinically and/or histologically, had been made within the
first 7 days of life, neonates were premature, and the placenta had been submitted
for histological examination. In those cases with ACA, CD34 immunohistochemistry and
Martius scarlet blue staining was performed. Medical records were reviewed for demographics,
clinical variables, and clinical outcomes. Statistical analysis was performed using
Fisher exact test.
Results In total, 21 cases met defined inclusion criteria (12 NEC, 8 SIP, and 1 clinically
indeterminate). Mean gestational age was 27 weeks. Median age of presentation was
5 days. Placental histology showed ACA in 16 of 21 cases (76.2%). Of those with ACA,
13 of 16 (81.3%) had umbilical phlebitis, 12 of 16 (75.0%) had umbilical arteritis,
6 of 16 (37.5%) funisitis, and 12 of 16 (75.0%) had chorionic vasculitis. No differences
(p > 0.05) were seen between ACA and diagnosis or clinical outcome (Fisher exact test).
Of the 16 cases, 14 with ACA that later developed either NEC or SIP showed vasculitis
in the umbilical cord and/or chorionic plate and/or stem villi vasculature. The association
between ACA and vasculitis was highly significant (p < 0.01). Of those with ACA on placental histology, 12 of 16 (75.0%) cases were found
to have intermediate-advanced stage fetal inflammatory response (FIR), whereas 13
of 16 (81.3%) had grade 2 (severe) FIR. Of the 16 cases, 8 (50.0%) had evidence of
fibrin deposition/early thrombus formation within placental and/or umbilical vasculature.
These were associated with vascular endothelial injury in vessels with prominent vasculitis.
Conclusion NEC or SIP shows a significant association with ACA with presence of vasculitis as
part of the FIR (p < 0.01). In a proportion of cases, the development of fibrin deposition in response
to vasculitic endothelial damage of the placental vasculature may form part of the
mechanism linking ACA and early postnatal development of NEC and/or SIP.
Keywords acute chorioamnionitis - necrotizing enterocolitis - spontaneous intestinal perforation
