Monitoring Biocatalytic Transformations Mediated by Polyketide Synthase Enzymes in Cell Lysate via Fluorine NMR

Shawn K. Piasecki; Adrian T. Keatinge-Clay

doi:10.1055/s-0032-1316554

Synlett, Inhaltsverzeichnis

Synlett 2012; 23(12): 1840-1842
DOI: 10.1055/s-0032-1316554

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Monitoring Biocatalytic Transformations Mediated by Polyketide Synthase Enzymes in Cell Lysate via Fluorine NMR

Authors

Shawn K. Piasecki

^aInstitute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA
Adrian T. Keatinge-Clay*

^aInstitute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA

^bDepartment of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station A5300, Austin, TX 78712, USA, Fax: +1(512)4718696 eMail: adriankc@mail.utexas.edu

Abstract

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Abstract

The biocatalytic employment of modular polyketide synthase enzymes in cell lysate has become a viable route to preparative quantities of synthetically valuable polyketide fragments. We report the quantitative, uninvasive, and continuous monitoring of such biocatalytic reactions by observing trifluoromethyl-bearing substrates via ¹⁹F NMR spectroscopic analysis. To demonstrate the utility of this technique, we followed reactions catalyzed by a thioesterase and several ketoreductases.

Key words

biosynthesis - biocatalysis - polyketide synthase enzymes - polyketide - fluorine NMR

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Referenzen

References

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