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DOI: 10.1055/a-1464-1525
Enhanced Skin Permeation of Punicalagin after Topical Application of Pluronic Micelles or Vesicles Loaded with Lafoensia pacari Extract
Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico Supported by: Fundação de Amparo à Pesquisa do Estado de Goiás Supported by: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Abstract
Punicalagin, the principal ellagitannin of Lafoensia pacari leaves, has proven antioxidant activity, and standardized extracts of L. pacari can be topically used for skin aging management. We hypothesized that Pluronic nanomicelles or vesicles could solubilize sufficiently large amounts of the standardized extracts of L. pacari and provide chemical stability to punicalagin. The standardized extracts of L. pacari were obtained with an optimized extraction procedure, and the antioxidant activity was characterized. Formulations containing Pluronic at 25% and 35% were obtained with or without Span 80. They were characterized by average diameter, polydispersity index, punicalagin content, physicochemical stability, and rheology. A release and skin permeation study was carried out in vertical diffusion cells. The extraction procedure allowed quantifying high punicalagin content (i.e., 141.61 ± 3.87 mg/g). The standardized extracts of L. pacari showed antioxidant activity for all evaluated methods. Pluronic at 25 and Pluronic at 35 with standardized extracts of L. pacari showed an average diameter of about 25 nm. The addition of Span 80 significantly increased the mean diameter by 15-fold (p < 0.05), indicating the spontaneous formation of vesicles. Pluronic formulations significantly protected punicalagin from chemical degradation (p < 0.05). Pluronic at 25 formulations presented as free-flowing liquid-like systems, while Pluronic at 35 resulted in an increase of about 44-fold in |ƞ*|. The addition of Span 80 significantly reduced the Pluronic sol-gel transition temperature (p < 0.05), indicating the formation of vesicles. Formulations with Span 80 significantly enhanced punicalagin skin permeation compared to formulations without Span 80 (p < 0.05). Formulations with Span 80 were demonstrated to be the most promising formulations, as they allowed significant permeation of punicalagin (about 80 to 315 µg/cm2), which has been shown to have antioxidant activity.
Key words
Lafoensia pacari - Lythraceae - antioxidant activity - supramolecular gels - topical administration - phytocosmeticSupporting Information
- Supporting Information
Comparison of the visual aspect of formulations was analyzed by visual inspection and are available (Fig. 1S) as Supporting information. Optical microscopy and the mean diameter (µm) of some formulations were obtained by laser diffraction (Mastersizer 3000, Malvern Instruments) (Fig. 2S). Viscosities of Pluronic dispersions with SELp as a function of shear rate are also available in Fig. 3S (Supporting information). pH measurements were carried out at 20 °C with a PG2000 pHmeter (Gehaka) immediately after preparation (T = 0) and after storage for 15 days (T = 15) and presented in Fig. 4S (Supporting information).
Publication History
Received: 23 October 2020
Accepted after revision: 22 March 2021
Article published online:
16 April 2021
© 2021. Thieme. All rights reserved.
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