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CC BY 4.0 · Revista Brasileira de Cirurgia Plástica (RBCP) – Brazilian Journal of Plastic Surgery 2024; 39(01): 217712352023rbcp0819pt
DOI: 10.5935/2177-1235.2023RBCP0819-PT
Artigo Original

Cryopreservation of adipose tissue-derived mesenchymal stem cells: An alternative to serial fat grafting in breast reconstruction

Article in several languages: português | English
MARCELO MORENO 
1   Universidade Federal da Fronteira Sul, Chapecó, SC, Brasil.
,
ELOISA LEHR FILIPPI CHIELLA 
2   Universidade Comunitária da Região de Chapecó, Chapecó, SC, Brasil.
,
RAPHAELA PASSUELLO 
2   Universidade Comunitária da Região de Chapecó, Chapecó, SC, Brasil.
,
ALINE MANICA 
2   Universidade Comunitária da Região de Chapecó, Chapecó, SC, Brasil.
,
JULIANA CRISTINA SCHMIDT 
2   Universidade Comunitária da Região de Chapecó, Chapecó, SC, Brasil.
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▪ ABSTRACT

Introduction:

Fat grafting is an autologous graft of cells from subcutaneous tissue, which can be used as a complementary technique in breast reconstruction. Given this, the cryopreservation of adipose tissue-derived mesenchymal stem cells (ADMSCs) could be a way to collect them in one surgical procedure and after performing fractional fat grafting. Dimethyl sulfoxide (DMSO) is a cryopreservative used in cell research, but it is potentially toxic, which would make it impossible to use cryopreserved ADMSCs in clinical practice. New cellular cryopreservatives, without toxicity, have been described in the experimental scientific literature, such as the substances L-proline and trehalose. Therefore, this work aimed to evaluate the viability of ADMSCs cryopreserved with the combination of L-proline and trehalose over up to 90 days.

Method:

Experimental study in which lipoaspirate samples were obtained from 9 patients. The cellular fraction was processed and frozen with L-proline (1.5M) + trehalose (0.2M) or with DMSO + fetal bovine serum (FBS) as control. After 30 and 90 days, the samples were thawed, and cell viability was assessed using the MTT technique.

Results:

The analysis of ADMSCs, after 1 and 3 months of freezing, indicated that samples treated with L-proline + trehalose showed similar viability to those preserved with DMSO and SFB (p=0.444).

Conclusion:

The association of L-proline and trehalose kept ADMSC viable for 30 and 90 days of freezing, and could be an alternative as a cellular cryopreservative without toxicity and enabling the use of serial fat grafting.

Keywords:

Trehalose - Lipectomy - Breast neoplasms - Mesenchymal stem cells - Adipocytes - Proline

Institution: Universidade Comunitária da Região de Chapecó (Unochapecó), Chapecó, SC, Brazil.




Publication History

Received: 15 May 2023

Accepted: 20 August 2023

Article published online:
20 May 2025

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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Bibliographical Record
MARCELO MORENO, ELOISA LEHR FILIPPI CHIELLA, RAPHAELA PASSUELLO, ALINE MANICA, JULIANA CRISTINA SCHMIDT. Criopreservação de células-tronco mesenquimais do tecido adiposo: Uma alternativa para lipoenxertia seriada na reconstrução mamária. Revista Brasileira de Cirurgia Plástica (RBCP) – Brazilian Journal of Plastic Surgery 2024; 39: 217712352023rbcp0819pt.
DOI: 10.5935/2177-1235.2023RBCP0819-PT
 

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