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CC BY 4.0 · Brazilian Journal of Oncology 2017; 13(45): e-BJO20171345A44
DOI: 10.26790/BJO20171345A44
Artigo Original

Correlação entre o polimorfismo (-1082) da interleucina-10 e o desenvolvimento de carcinomas de pele

Correlation between polymorphism (-1082) of interleukin-10 and the injury of development cancer of skin
Daniela Rigo
1   Universidade do Oeste de Santa Catarina, São Miguel do Oeste, SC, Brasil
,
Giovana Weber
1   Universidade do Oeste de Santa Catarina, São Miguel do Oeste, SC, Brasil
,
Luciane Franz
1   Universidade do Oeste de Santa Catarina, São Miguel do Oeste, SC, Brasil
,
Gustavo Borba de Miranda
2   Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, Rio Grande, RS, Brasil
,
Everton Boff
1   Universidade do Oeste de Santa Catarina, São Miguel do Oeste, SC, Brasil
,
Flávia Hoffmann Palu
1   Universidade do Oeste de Santa Catarina, São Miguel do Oeste, SC, Brasil
,
Eduardo Ottobelli Chielle
1   Universidade do Oeste de Santa Catarina, São Miguel do Oeste, SC, Brasil
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RESUMO

Objetivo: O câncer de pele é o mais frequente no Brasil especialmente em Santa Catarina. A interleucina-10 (IL-10) é uma citocina imunossupressora que pode possibilitar a fuga das células cancerosas do sistema imune, deste modo, polimorfismos no gene da IL-10 têm sido implicados na susceptibilidade e desenvolvimento de neoplasias. Este estudo teve como objetivo analisar as frequências alélicas e genotípicas do gene da IL-10 e sua associação com carcinomas de pele.

Métodos: Tratou-se de um estudo caso-controle onde foram analisadas 90 amostras, sendo o grupo teste composto por 49 tecidos de epiderme com carcinoma e o grupo controle por 41 raspados de mucosa oral de indivíduos sadios. A técnica ARMS-PCR foi utilizada para a identificação do polimorfismo.

Resultados: A frequência alélica para o grupo controle foi de 70% para o alelo A e 30% para o G; no grupo teste obteve-se 44% para o alelo A e 56% para o alelo G. A frequência genotípica observada no grupo controle foi AA (42%), AG (56%) e GG (25), e no grupo teste AA (24%), AG (39%) e GG (37%). Houve uma diferença estatística entre os grupos, tanto para a frequência alélica, quanto para o genótipo (p<0,05).

Conclusão: Observou-se uma maior prevalência do alelo G e dos genótipos AG e GG no grupo teste, quando comparado com o grupo controle, fator que poderia ser um adjuvante no desenvolvimento de carcinomas da epiderme. Este resultado demonstra a importância da resposta imune no desenvolvimento no processo de transformação de malignidade.

ABSTRACT

Objective: Skin cancer is the most common in Brazil especially in Santa Catarina. Inter-leukin-10 (IL-10) is an immunosuppressive cytokine that can allow the escape of cancer cells of the immune system, thereby polymorphisms in the IL-10 gene have been implicated in susceptibility and the development of malignancies. This study aimed to analyze the frequencies of the alleles and genotypic at position -1082 of the promoter region of IL-10 and its association with skin carcinomas.

Methods: This was a case-control study which analyzed 90 samples, and the test group of 49 skin tissue carcinoma and the control group by 41 scrapings oral mucosa of healthy individuals. The ARMS-PCR was used to identify the polymorphism.

Results: The allelic frequency for the control group was 70% for the A allele and 30% for G; in the test group gave 44% to 56% allele A and allele G Genotype frequency observed in the control group was AA (42%), AG (56%) and GG (25), while in group test was AA (24%), AG (39%) and GG (37%). There was a statistical difference between groups for both the allelic frequency, as for genotype (p <0.05).

Conclusion: There was a higher prevalence of the G allele and genotype AG and GG in the test group compared to the control group, a factor that could be an adjuvant in the development of skin carcinomas. This result demonstrates the importance of developing immune response in the malignant transformation process.

Descritores:

Imunossupressão - Interleucina-10 - Polimorfismo - Carcinoma epithelial

Keywords:

Immunosuppression - Interleukin-10 - Polymorphism - Epithelial carcinoma


Publication History

Received: 16 February 2017

Accepted: 24 September 2017

Article published online:
07 March 2025

© 2017. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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Bibliographical Record
Daniela Rigo, Giovana Weber, Luciane Franz, Gustavo Borba de Miranda, Everton Boff, Flávia Hoffmann Palu, Eduardo Ottobelli Chielle. Correlação entre o polimorfismo (-1082) da interleucina-10 e o desenvolvimento de carcinomas de pele. Brazilian Journal of Oncology 2017; 13: e-BJO20171345A44.
DOI: 10.26790/BJO20171345A44
 

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