Eur J Pediatr Surg 2007; 17(6): 416-419
DOI: 10.1055/s-2007-989218
Original Article

© Georg Thieme Verlag KG Stuttgart · New York

Intestinal Prostaglandin E2 Expression in Rats with Obstructive Jaundice

A. Aslan1 , M. Bulbul2 , O. Elpek3 , C. Karaveli1 , N. Izgut-Uysal2
  • 1Department of Pediatric Surgery, Akdeniz University School of Medicine, Antalya, Turkey
  • 2Department of Physiology, Akdeniz University School of Medicine, Antalya, Turkey
  • 3Department of Pathology, Akdeniz University School of Medicine, Antalya, Turkey
Further Information

Publication History

received May 14, 2007

accepted May 23, 2007

Publication Date:
11 December 2007 (online)

Abstract

Aim: How obstructive jaundice causes the intestinal barrier to be injured is still controversially discussed. In this study, we hypothesize that intestinal prostaglandin E2, a cytoprotective factor, may be affected by the bile duct obstruction. Materials and Methods: Four groups of Wistar-Albino rats were used: in Groups 1 and 3, the rats underwent a sham operation. In Groups 2 and 4, the common bile duct was doubly ligated. Relaparotomy was performed after one week in Groups 1 and 2, and after two weeks in Groups 3 and 4, and specimens of the jejunum, ileum and liver were obtained for intestinal PGE2 analysis and histopathological evaluation. Results: Jejunal and ileal PGE2 levels had significantly decreased in two-week bile duct-ligated rats compared to one-week ligated rats and the sham group (p < 0.01). Tissue injury scores (Chiu score) of the ileum were significantly higher in the two-week and one-week ligated rats than in the controls (p < 0.01 and p < 0.05, respectively). The jejunal injury score was significantly higher in the two-week ligated rats compared to controls (p < 0.05). The ileal and jejunal injury scores were higher in the two-week ligated rats than in the one-week ligated rats (p < 0.01 and p < 0.05, respectively). Precirrhotic fibrosis was detected in all two-week ligated rats, but in only 7 of 10 one-week ligated rats. Conclusions: Obstructive jaundice associated with intestinal tissue injury and precirrhotic changes leads to reduced intestinal PGE2-levels, suggesting an adverse effect on the intestinal cytoprotective process.

References

Dr. Adnan Aslan

Akdeniz Universitesi Tip Fakültesi
Cocuk Cerrahisi Anabilim Dali

07070 Antalya

Turkey

Email: [email protected]