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Synthesis 2001; 2001(4): 0565-0572
DOI: 10.1055/s-2001-12355
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Preparation of Spin-Labeled-2-Amino-dA, dA, dC and 5-Methyl-dC Phosphoramidites for the Automatic Synthesis of EPR Active Oligonucleotides

Cesare Giordano* , Federica Fratini, Donato Attanasio, Luciano Cellai
  • *Centro di Studio per la Chimica del Farmaco, CNR, Dipartimento di Studi Farmaceutici, Università "La Sapienza", P.le A Moro 5, 00185, Rome, Italy; Fax + 39(6)49 14 91; E-mail: cesare.giordano@uniroma1.it
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Publication Date:
31 December 2001 (online)

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2,2,6,6-Tetramethyl-1-piperidinyloxy free radical (TEMPO) labeled phosphoramidites of 2-amino-dA, dA, dC and 5-methyl-dC were synthesized and used for the automatic synthesis of mono-labeled oligodeoxynucleotides (ODNs), which proved active to EPR. It is now possible to insert a paramagnetic probe into nucleic acids in a site- and type-specific manner. The combination of this synthetic approach with EPR spectroscopy can be exploited for performing studies on dynamics and local structural modifications in nucleic acids.

DNA - nucleosides - ODNs - nitroxides - EPR

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