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Synlett 1990; 1990(11): 709-710
DOI: 10.1055/s-1990-21221
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Preparative Enzymatic Conversion of Guanosine-5′ -monophosphate to Guanosine-5′ - triphosphate

Regine Stiller* , Joachim Thiem
  • *Institut für Organische Chemie, Universität Hamburg, Martin-Luther-King-Platz 6, D-2000 Hamburg 13, Germany
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Publikationsdatum:
08. März 2002 (online)

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An enzyme reactor containing guanylate kinase (EC 2.7.4.8) and pyruvate kinase (EC 2.7.1.40) was used for the preparative synthesis of guanosine-5′ -triphosphate (GTP) starting from guanosine-5′ -monophosphate (GMP), phospho(enol)-pyruvate (PEP) [2-(phosphonooxy)-2-propenoic acid] and catalytic amounts of adenosine-5′ -triphosphate (ATP).