Synthesis of a Ubiquitinated Histone Mimic Bearing a New Thioether Linkage
Received: 17 January 2017
Accepted after revision: 19 February 2017
09 March 2017 (eFirst)
Published as part of the Cluster Recent Advances in Protein and Peptide Synthesis
Current studies on histone ubiquitination require the development of more effective approaches for the preparation of ubiquitinated histones. Here, we report a new approach to obtain ubiquitinated histone mimics by using a thioether linkage. The synthetic ubiquitinated histone mimic is stable under reducing conditions and has the same atom number as the native structure. Biochemical experiments showed that the mimic is a good surrogate for natural ubiquitinated histone.