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DOI: 10.1055/a-1197-4293
Mütter-Screening in einem COVID-19-Niedrig-Pandemiegebiet: Bestimmung SARS-CoV-2-spezifischer Antikörper bei 401 Rostocker Müttern mittels ELISA und Immunfluoreszenz-Bestätigungstest
Screening of Mothers in a COVID-19 Low-Prevalence Region: Determination of SARS-CoV-2 Antibodies in 401 Mothers from Rostock by ELISA and Confirmation by Immunofluorescence
Zusammenfassung
Hintergrund Bei Kindern verläuft die Infektion mit SARS-CoV-2, dem Erreger von COVID-19, in der Regel weniger schwer und häufiger symptomlos als bei Erwachsenen. Kinder können aber diese Infektion u. a. auf vulnerable Erwachsene übertragen. Daher sind zur Zeit Einrichtungen der Kinderbetreuung und Schulen teilweise geschlossen.
Methoden Da sich der Infektionsstatus bei Kindern unter 13 Jahren und deren Müttern meist ähnlich darstellt, untersuchten wir bei 401 Rostocker Müttern von Kindern zwischen 1 und 10 Jahren Rachenabstriche auf SARS-CoV-2 und Serumproben auf Antikörper gegen diesen Erreger.
Ergebnisse Wir fanden bei keiner der untersuchten Mütter RNA von SARS-CoV-2 im Rachenabstrich. Im ELISA waren IgG-Antikörper in einem Serum positiv, IgA-Antikörper waren in 11 Seren positiv und in 3 Seren grenzwertig. Alle 401 Seren waren im Fluoreszenz-Bestätigungstest (IIFT) mit FITC-markierten IgG-, IgA- und IgM-Antikörpern negativ.
Schlussfolgerung Im Untersuchungszeitraum waren bei den getesteten Rostocker Müttern weder RNA von SARS-CoV-2 noch spezifische Antikörper nachweisbar.
Abstract
Background In children, the infection with SARS-CoV-2, the cause of COVID-19, tends to be clinically inapparent more often or less severe than in adults. The spread of this infection from children poses a danger to vulnerable adults. Therefore, child care institutions and schools currently are widely closed.
Methods Since the status of infection tends to be congruent in mothers and their children, we tested 401 mothers of children between 1 and 10 years in the city of Rostock (State of Mecklenburg-Westpomerania, northeast of Germany), for the presence of RNA of SARS-CoV-2 in throat swabs, and of antibodies against SARS-CoV-2 in serum.
Results In none of the mothers tested, RNA of this virus was detected in the throat swab. In the ELISA test, IgG antibodies were positive in one serum sample, IgA antibodies were positive in 11, and borderline in 3 serum samples. All 401 sera were negative in the indirect immunofluorescence test (IIFT) with FITC-labeled IgG, IgA, und IgM antibodies.
Conclusion At the time of this study, neither SARS-CoV-2 RNA, nor specific antibodies against SARS-CoV-2 were detectable in the mothers tested in Rostock.
Publication History
Article published online:
22 June 2020
© .
© Georg Thieme Verlag KG
Stuttgart · New York
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Literatur
- 1 Dong Y, Mo X, Hu Y. et al Epidemiology of COVID-19 among children in China. Pediatrics 2020; 145: e2020-e0702 . doi:10.1542/peds.2020-0702
- 2 Choi SH, Kim HW, Kam JM. et al Epidemiology and clinical features of coronavirus disease 2019 in children. CEP 2020; 63: 125-132 . doi:10.3345/cep.2020.00535
- 3 Han YN, Feng ZW, Sun LN. et al A comparative-descriptive analysis of clinical characteristics in 2019-coronavirus-infected children and adults. J Med Virol 2020; 92: 1-7 . doi:10.1002/jmv.25835
- 4 Wang D, Ju XL, Xie F. et al Clinical analysis of 31 cases of 2019 novel coronavirus infection in children from six provinces (autonomous region) of northern China. Zhonghua Er Ke Za Zhi 2020; 58: 269-274 . doi:10.3760/cma.j.cn112140-20200225-00138
- 5 Streeck H, Schulte B, Kümmerer BM. et al Infection fatality rate of SARS-CoV-2 infection in a German community with a super-spreading event. 04. Mai 2020. abgerufen am 19.05.2020. https://www.ukbonn.de/C12582D3002FD21D/vwLookupDownloads/Streeck_et_al_Infection_fatality_rate_of_SARS_CoV_2_infection2.pdf/%24FILE/Streeck_et_al_Infection_fatality_rate_of_SARS_CoV_2_infection2.pd
- 6 Krüttgen A, Cornelissen CG, Dreher M. et al Comparison of four new commercial serologic assays for determination of SARS-CoV-2 IgG. Journal of Clinical Virology 2020; 128: 104394 . doi:10.1016/j.jcv.2020.104394
- 7 Tamburello A, Marando M. Immunoglobulins or convalescent plasma to tackle COVID-19: buying time to save lives – current situation and perspectives. Swiss Med Wkly 2020; 150: w20264 . doi:10.4414/smw.2020.20264. eCollection 2020 Apr 20
- 8 Wölfel R, Corman VM, Guggemos W. et al Virological assessment of hospitalized patients with COVID-19. Nature 2020; 581: 465-469 . doi:10.1038/s41586-020-2196-x
- 9 Kumar M, Mazur S, Ork BL. et al. Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus. J Virol. Methods 2015; 223: 13-18
- 10 Emmerich P, Thome-Bolduan C, Drosten C. Reverse ELISA for IgG and IgM antibodies to detect Lassa virus infections in Africa. J Clin Virol 2006; 37: 277-281 . doi:10.1016/j.jcv.2006.08.015
- 11 Gresner AM, Arndt T. (Hrsg.). Lexikon der Medizinischen Laboratoriumsdiagnostik, Lexikon der Autoimmundiagnostik. 2. Auflage. Springer-Verlag Heidelberg; 2013: 198-199
- 12 Cowling BJ, Ali ST, Ng TWY. et al Impact assessment of non-pharmaceutical interventions against coronavirus disease 2019 and influenza in Hong Kong: an observational study. Lancet Public Health 2020; 5: e279-e288 . doi:10.1016/S2468-2667(20)30090-6
- 13 Jones TC, Mühlemann B, Veith T. et al An Analysis of SARS-CoV-2 viral load by patient age. 29.04.2020. heruntergeladen am 19.05.2020. https://virologie-ccm.charite.de/fileadmin/user_upload/microsites/m_cc05/virologie-ccm/dateien_upload/Weitere_Dateien/analysis-of-SARS-CoV-2-viral-load-by-patient-age-v2.pdf
- 14 Zachariah P, Halabi KC, Johnson CL. et al Symptomatic Infants have Higher Nasopharyngeal SARS-CoV-2 Viral Loads but Less Severe Disease than Older Children. Clin Infect Dis 2020; 66: ciaa608 . doi:10.1093/cid/ciaa608
- 15 Zimmermann P, Curtis N. Coronavirus Infections in Children Including COVID-19: An Overview of the Epidemiology, Clinical Features, Diagnosis, Treatment and Prevention Options in Children. The Pediatric Infectious Disease Journal 2020; 39: 355-368
- 16 Mehta NS, Mytton OT, Mullins EWS. et al SARS-CoV-2 (COVID-19): What do we know about children? A systematic review. Clin Infect Dis 2020; 66: ciaa556 . doi:10.1093/cid/ciaa556
- 17 Bi Q, Wu Y, Mei S. et al Epidemiology and Transmission of COVID-19 in Shenzhen China: Analysis of 391 cases and 1.286 of their close contacts. Lancet Infect Dis 2020; 20 in press. pii: S1473-3099(20)30287-5. DOI: 10.1016/S1473-3099(20)30287.
- 18 Zhang J, Litvinova M, Liang Y. et al Changes in contact patterns shape the dynamics of the COVID-19 outbreak in China. Science 2020; 364: eabb8001 . doi:10.1126/science.abb8001
- 19 Danis K, Epaulard O, Bénet T. et al. Cluster of Coronavirus Disease (COVID-19) in the French Alps, 2020. Clin Infect Dis 2020; 69: ciaa424 https://doi.org/10.1093/cid/ciaa424