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Tierarztl Prax Ausg G Grosstiere Nutztiere 2016; 44(05): 287-294
DOI: 10.15653/TPG-160214
Original Article
Schattauer GmbH

Ovarian response and conception rate following oestrus synchronization using three protocols in Egyptian buffalo heifers

Ovarielle Reaktion und Konzeptionsrate nach Östrussynchronisation mittels drei verschiedener Protokolle bei ägyptischen Büffelfärsen
Hassan A. Hussein
1   Theriogenology Department, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
,
Ragab H. Mohamed
2   Theriogenology Department, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt
,
Mohamed Hossam
3   Ard-El-khair Buffalo Farm, Abnob, Assiut, Egypt
,
Axel Wehrend
4   Clinic for Obstetrics, Gynaecology and Andrology of Large and Small Animals, Justus Liebig University, Giessen, Germany
› Author Affiliations
Further Information

Publication History

Received: 20 February 2016

Accepted after major revision: 28 June 2016

Publication Date:
23 December 2017 (online)

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Summary

Objective: The aim of this study was to monitor the ovarian response and conception rate following estrous synchronization using CIDR, Ovsynch and double prostaglandin F2α protocols in Egyptian buffalo heifers. Material and methods: A total of 80 cyclic buffalo heifers were divided into four equal groups: CIDR (intravaginal progesterone releasing device, EAZI-BREEDTM CIDR®), Ovsynch (GnRH, PGF2α, GnRH injections), PGF (double PGF2α doses) and control. Timed artificial insemination (TAI) was performed in all heifers. All animals were exa - mined using ultrasound and blood samples were collected for measurement of progesterone. Results: A new follicular wave occurred earlier in the Ovsynch and PGF groups than in the CIDR group (p < 0.05). The mean diameter of the ovulatory follicle was smaller in the CIDR group than in the Ovsynch and PGF groups (p < 0.05). The ovulation rate was 100% in the CIDR group, 75% in the Ovsynch group and 70% in the PGF group. In the control group a lower pregnancy rate (20%) was determined in than in the CIDR (35%), Ovsynch (40%) and PGF (35%) groups. Progesterone concentrations were numerically higher in pregnant heifers of the CIDR group but the difference was non-significantly compared to the Ovsynch, PGF and control groups (p > 0.05). Conclusion and clinical relevance: EAZIBREEDTM CIDR®, Ovsynch-based TAI and PGF protocols were effective in synchronizing oestrus and resulted in nearly similar pregnancy rates in Egyptian buffalo heifers.

Zusammenfassung

Ziel dieser Studie war, bei ägyptischen Büffelfärsen die Reaktion der Ovarien und die Konzeptionsrate nach Östrussynchronisation mit CIDR, Ovsynch und zweimaliger PGF2α-Gabe vergleichend zu erfassen. Material und Methoden: Insgesamt 80 zyklische Büffelfärsen wurden in vier gleich große Gruppen eingeteilt: CIDR-Gruppe (intravaginale progesteronfreisetzende Spange, EAZI-BREEDTM CIDR®), Ovsynch- Gruppe (GnRH, PGF2α, GnRH-Injektion), PGF-Gruppe (zweimalige Gabe von PGF2α) und Kontrollgruppe. Alle Tiere wurden terminorientiert besamt. Bei allen Tieren erfolgten eine ultrasonographische Untersuchung und eine Blutentnahme zur Progesteronbestimmung. Ergebnisse: Eine neue Follikelwelle trat in der Ovsynch- und PGFGruppe früher auf als in der CIDR-Gruppe (p < 0,05). Der mittlere Durchmesser des ovulatorischen Follikel war in der CIDR-Gruppe kleiner als in der Ovsynch- und PGF-Gruppe (p < 0,05). Die Ovulationsrate betrug in der CIDR-Gruppe 100%, in der Ovsynch-Gruppe 75% und in der PGF-Gruppe 70%. In der Kontrollgruppe ergab sich eine niedrigere Trächtigkeitsrate (20%) als in der CIDR- (35%), Ovsynch-(40%) und PGF-Gruppe (35%). Die Progesteronkonzentration lag in der CIDRGruppe höher als in der Ovsynch-, PGF- und Kontrollgruppe, doch war der Unterschied nicht signifikant (p > 0,05). Schlussfolgerung und klinische Relevanz: CIDR-, Ovsynch- und PGF-Protokolle erwiesen sich als wirksam in der Brunstsynchronisation von ägyptischen Büffel - färsen und führten zu fast gleich hohen Trächtigkeitsraten.

keywords

Reproduction - CIDR - Ovsynch - PGF2α

Schlüsselworter

Reproduktion - CIDR - Ovsynch - PGF2α
 

  • Literatur

  • 1 Ahmad N, Schrick FN, Butcher RL, Inskeep EK. Effect of persistent follicles on early embryonic losses in beef cows. Biol Reprod 1995; 52: 1129-1135.
    CrossrefPubMedGoogle Scholar
  • 2 Ali A, Fahmy S. Ovarian dynamics and milk progesterone concentrations in cycling and non-cycling buffalo-cows (Bubalus bubalis) during Ovsynch program. Theriogenology 2007; 68: 23-28.
    CrossrefPubMedGoogle Scholar
  • 3 Barile VL, Galasso A, Carretta A. Preliminary results on conception rates in Italian buffaloes after use of intravaginal progesterone device and artificial insemination.. Buffalo letter (F.A.O. Bulletin, Rome) 1996; 6 3-4 (02) 433-440.
    PubMedGoogle Scholar
  • 4 Barile VL, Galasso A, Marchiori E, Borghese A. Effect of PRID treatment on oestrus synchronisation and progesterone in Italian buffaloes. Proceeding of V World Buffalo Congress. Caserta, Italy: 13-16 October 1997: 738-743.
    Google Scholar
  • 5 Barile VL. Improving reproductive efficiency in female buffaloes. Livestock Prod. Sci. 2005; 92: 183-194.
    CrossrefPubMedGoogle Scholar
  • 6 Bartolomeu CC, Del Rei AJM, Madureira EH, Souza AJ, Silva AO, Baruselli PS. Timed insemination using synchronization of ovulation in buffaloes using CIDR-B, CRESTAR and Ovsynch. Anim Breed Abstr 2002; 70: 332.
    PubMedGoogle Scholar
  • 7 Bartolomeu CC, Del Rei AJ, Álvares CTG, Vilar GD. Follicular dynamics during synchronization of ovulation of nuliparous buffaloes cows during unfavourable reproductive station. Ital J Anim Sci 2007; 6 (Suppl. 02) 589-592.
    CrossrefPubMedGoogle Scholar
  • 8 Berber RC de A, Madureira EH, Baruselli PS. Comparison of two Ovsynch protocols (GnRH versus LH) for fixed-timed insemination in buffalo (Bubalus bubalis). Theriogenology 2002; 57: 1421-1430.
    CrossrefPubMedGoogle Scholar
  • 9 Bhalaru SS, Tiwana MS, Singh N. Effect of body condition at calving on subsequent reproductive performance in buffaloes. Indian J Anim Sci 1987; 57: 33-36.
    PubMedGoogle Scholar
  • 10 Borghese A. In: FIL/IDF Bulletin of the International Dairy Federation No. 438. The World Dairy Situation. Brussels (Belgium): 2009: 593-604.
    Google Scholar
  • 11 Campanile G, Neglia G, Gesparrini B, Galieno G, Prandi A, Diblo R. Embryonic mortality in buffaloes synchronized and mated by AI during the seasonal decline in reproductive function. Theriogenology 2005; 63: 2334-2340.
    CrossrefPubMedGoogle Scholar
  • 12 Campanile G, Di Palo R, Neglia G, Vecchio D, Gasparrini B, Prandi A, Galiero G, D’Occhio MJ. Corpus luteum function and embryonic mortality in buffaloes treated with a GnRH agonist, hCG and progesterone. Theriogenology 2007; 67: 1393-1398.
    CrossrefPubMedGoogle Scholar
  • 13 Campanile G, Vecchio D, Di Palo R, Neglia G, Gasparrini B, Prandi A, Zicarelli L, D’Occhio MJ. Delayed treatment with GnRH agonist, hCG and progesterone and reduced embryonic mortality in buffaloes. Theriogenology 2008; 12: 127-131.
    PubMedGoogle Scholar
  • 14 Chaikhun T, Tharasanit T, Rattanatep J, De Rensis F, Techakumphu M. Fertility of swamp buffalo following the synchronization of ovulation by the sequential administration of GnRH and PGF2alpha combined with fixedtimed artificial insemination. Theriogenology 2010; 74: 1371-1376.
    CrossrefPubMedGoogle Scholar
  • 15 Chohan KR. Estrus synchronization with lower dose of PGF 2alpha and subsequent fertility in subestrous buffalo. Theriogenology 1999; 50: 1101-1108.
    PubMedGoogle Scholar
  • 16 Colazo MG, Kastelic JP, Mapletoft RJ. Effects of estradiol cypionate (ECP) on ovarian follicular dynamics, synchrony of ovulation, and fertility in CIDR-based fixed-time AI programs in beef heifers. Theriogenology 2003; 60: 855-865.
    CrossrefPubMedGoogle Scholar
  • 17 Colazo MG, Kastelic JP, Whittaker PR, Gavaga QA, Wilde R, Mapletoft RJ. Fertility in beef cattle given a new or previously used CIDR insert and estradiol, with or without progesterone. Anim Reprod Sci 2004; 81: 25-34.
    CrossrefPubMedGoogle Scholar
  • 18 De Rensis F, Ronci G, Guarneri P, Xuan Nguyen B, Presicce GA, Huszenicza G, Scaramuzzi RJ. Conception rate after fixed time insemination following Ovsynch protocol with and without progesterone supplementation in cyclic and non-cyclic Mediterranean Italian buffaloes (Bubalus bubalis). Theriogenology 2005; 63: 1824-1831.
    CrossrefPubMedGoogle Scholar
  • 19 Drost M. Bubaline versus bovine reproduction. Theriogenology 2007; 68: 447-449.
    CrossrefPubMedGoogle Scholar
  • 20 El-Wishy AB. The postpartum buffalo. I. Endocrinological changes and uterine involution. Theriogenology 2007; 97: 201-215.
    PubMedGoogle Scholar
  • 21 Ginther OJ, Kastelic JP, Knopf L. Temporal associations among ovarian events in cattle during oestrous cycles with two and three follicular waves. J Reprod Fert 1989; 87: 223-230.
    CrossrefPubMedGoogle Scholar
  • 22 Ginther OJ, Kot K, Kulick LJ, Wiltbank MC. Emergence and eviation of follicles during the development of follicular waves in cattle. Theriogenology 1997; 48: 75-87.
    CrossrefPubMedGoogle Scholar
  • 23 Gür S, Türk G, Demirci E, Yüce A, Sönmez M, Özer Ş, Aksu EH. Effect of pregnancy and foetal number on diameter of corpus luteum, maternal progesterone concentration and oxidant/antioxidant balance in ewes. Reprod Domest Anim 2011; 46: 289-295.
    CrossrefPubMedGoogle Scholar
  • 24 Hattab SA, Kadoom AK, Palme R, Bamberg E. Effect of CRESTAR on estrus synchronization and the relationship between fecal and plasma concentrations of progestagens in buffalo cows. Theriogenology 2000; 54: 1007-1017.
    CrossrefPubMedGoogle Scholar
  • 25 Hussein HA. Untersuchungen zur ovariellen Reaktion im Rahmen der Zyklussynchronisation mittels GnRH/PGF2 alpha und deren Graviditätsresultat bei Milchrindern. Ph.D. thesis. Justus-Liebig-Univerisity; Giessen, Germany: 2003
    Google Scholar
  • 26 Hussein HA, Derar R, Fahmy S, El-Sherry TM, Megahed G. Ovarian response and progesterone profile during Ovsynch protocol in heifers and mixed parity buffaloes cows (Bubalus bubalis). 10th Sci. Cong. Egyptian Society for cattle diseases. 13-15 December 2009. Assiut (Egypt): 368-378.
    Google Scholar
  • 27 Kim U-H, Suh G-H, Nam H-W, Kang H-G, Kim H. Follicular wave emergence, luteal function and synchrony of ovulation following GnRH or estradiol benzoate in a CIDR-treated, lactating Holstein cows. Theriogenology 2005; 63: 260-268.
    CrossrefPubMedGoogle Scholar
  • 28 Lamb GC, Stevenson JS, Kesler DJ, Garverick HA, Brown DR, Salfen BE. Inclusion of an intravaginal progesterone insert plus GnRH and prostaglandin F2a for ovulation control in postpartum suckled beef cows. J Anim Sci 2001; 79: 2253-2259.
    CrossrefPubMedGoogle Scholar
  • 29 Mann GE, Lamming GE. The influence of progesterone during early pregnancy in cattel. Reprod Domest Anim 1999; 34: 269-274.
    CrossrefPubMedGoogle Scholar
  • 30 Martinez MF, Kastelic JP, Colazo MG, Mapletoft RJ. Effects of estradiol on gonadotrophin release, estrus and ovulation in CIDR-treated beef cattle. Domest Anim Endocrinol 2007; 33: 77-90.
    CrossrefPubMedGoogle Scholar
  • 31 Naseer Z, Ahmad E, Singh J, Ahmad N. Fertility following CIDR based synchronization regimens in anoestrous Nili-Ravi buffaloes. Reprod Domest Anim 2011; 46: 814-817.
    CrossrefPubMedGoogle Scholar
  • 32 Neglia G, Gasparrini B, Palo RD, Rosa CD, Zicarelli L, Campanile G. Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranean buffalo cows. Theriogenology 2003; 60: 125-133.
    CrossrefPubMedGoogle Scholar
  • 33 Paul V, Prakash BS. Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah buffaloes (Bubalus bubalis). Theriogenology 2005; 64: 1049-1060.
    CrossrefPubMedGoogle Scholar
  • 34 Peterson C, Alkar A, Smith S, Kerr S, Hall JB, Moore D, Kasimanickam R. Effects of one versus two doses of prostaglandin F2alpha on AI pregnancy rates in a 5-day, progesterone-based, CO- Synch protocol in crossbred beef heifers. Theriogenology 2011; 75: 1536-1542.
    CrossrefPubMedGoogle Scholar
  • 35 Pierson RH, Ginther OJ. Follicular populations during the estrous cycle in heifers. 1. Influence of day. Anim Reprod Sci 1987; 14: 165-176.
    CrossrefPubMedGoogle Scholar
  • 36 Presicce GA, Senatore EM, Bella A, De Santis G, Barile VL, De Mauro GJ, Terzano GM, Steccob R, Parmeggiani A. Ovarian follicular dynamics and hormonal pro_les in heifer and mixed-parity Mediterranean Italian buffaloes (Bubalus bubalis) following an estrus synchronization protocol. Theriogenology 2004; 61: 1343-1355.
    CrossrefPubMedGoogle Scholar
  • 37 Pursley JR, Mee MO, Wiltbank MC. Synchronization of ovulation in dairy cows using PGF2a and GnRH. Theriogenology 1995; 44: 915-923.
    CrossrefPubMedGoogle Scholar
  • 38 Pursley JR, Wiltbank MC, Stevenson JS, Ottobre JS, Varverick HA, Anderson LL. Pregnancy rates per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J Dairy Sci 1997; 80: 295-300.
    CrossrefPubMedGoogle Scholar
  • 39 Roche JF, Austin EJ, Ryan M, O’Rourke M, Mihm M, Diskin MG. Regulation of follicle waves to maximize fertility in cattle. J Reprod Fert 1999; (Suppl) 54: 61-71.
    PubMedGoogle Scholar
  • 40 Schmitt EJP, Diaz T, Drost M, Thatcher WW. Use of a Gonadotropin-releasing hormone agonist or human chorionic gonadotropin for timed insemination in cattle. J Anim Sci 1996; 74: 1084-1091.
    CrossrefPubMedGoogle Scholar
  • 41 Singh J, Nanda AS, Adams GP. The reproductive pattern and efficiency of female buffaloes. Anim Reprod Sci. 2000 60-61. 593-604.
    PubMedGoogle Scholar
  • 42 Singh C. Response of anestrus rural buffaloes (Bubalus bubalis) to intravaginal progesterone implant and PGF2α injection in summer. J Vet Sci 2003; 4: 137-141.
    PubMedGoogle Scholar
  • 43 SPSS for Windows. Personal package.2007 version 16.0.
    PubMed
  • 44 Xu ZZ, Verkerk GA, Mee JF, Morgan SR, Clark BA, Burke CR. Progesterone and follicular changes in postpartum noncyclic dairy cows after treatment with progesterone and estradiol or with progesterone GnRH PGF2a and estradiol. Theriogenology 2000; 54: 273-282.
    CrossrefPubMedGoogle Scholar
  • 45 Zaabel SM, Hega AO, Montasser AE, El-Sheikh H. Reproductive performance of anestrous buffaloes treated with CIDR. Anim Reprod 2009; 6: 460-464.
    PubMedGoogle Scholar
  • 46 Zerbe H, Gregory C, Grunert E. Zur Behandlung ovariell bedingter Zyklusstorungen beim Milchrind mit Progesteron-abgebenden Vorrichtungen. Tierarztl Umsch 1999; 54: 189-192.
    PubMedGoogle Scholar