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Tierarztl Prax Ausg G Grosstiere Nutztiere 2016; 44(05): 307-312
DOI: 10.15653/TPG-160047
Kasuistik
Schattauer GmbH

Nachweis von Weissella spp. in Viertelgemelksproben von zwei Milchkühen mit klinischer Mastitis

Ein FallberichtDetection of Weissella spp. in milk samples of two dairy cows with clinical mastitisA case report
Regina Wald
1   Universitätsklinik für Wiederkäuer, Department für Nutztiere und öffentliches Gesundheitswesen in der Veterinärmedizin, Veterinärmedizinische Universität Wien, Österreich
,
Martina Baumgartner
1   Universitätsklinik für Wiederkäuer, Department für Nutztiere und öffentliches Gesundheitswesen in der Veterinärmedizin, Veterinärmedizinische Universität Wien, Österreich
,
Verena Urbantke
1   Universitätsklinik für Wiederkäuer, Department für Nutztiere und öffentliches Gesundheitswesen in der Veterinärmedizin, Veterinärmedizinische Universität Wien, Österreich
,
Thomas Wittek
1   Universitätsklinik für Wiederkäuer, Department für Nutztiere und öffentliches Gesundheitswesen in der Veterinärmedizin, Veterinärmedizinische Universität Wien, Österreich
,
Beatrix Stessl
2   Institut für Milchygiene, Department für Nutztiere und öffentliches Gesundheitswesen in der Veterinärmedizin, Veterinärmedizinische Universität Wien, Österreich
› Author Affiliations
Further Information

Publication History

Eingegangen: 08 January 2016

Akzeptiert nach Revision: 11 April 2016

Publication Date:
23 December 2017 (online)

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Zusammenfassung

Dieser Fallbericht beschreibt die Isolierung und Differenzierung von Weissella (W.) spp. aus der Milch zweier Kühe mit klinischer Mastitis (Milchveränderung, Euterschwellung und Zellzahlerhöhung). Viertelgemelksproben von zwei Milchkühen (A und B) aus unterschiedlichen Betrieben wurden an das Milchlabor der Universitätsklinik für Wiederkäuer in Wien zur bakteriologischen Untersuchung eingesendet. Es wurden α-hämolysierende, Katalase-negative, grampositive Kokken in Reinkultur auf Columbia-Blut-Agar isoliert, die sich keiner Lancefield-Gruppe zuordnen ließen. Die biochemische Charakterisierung der Isolate (API® 20 Strep, bioMérieux) ergab Leuconostoc spp. Eine Kontrolluntersuchung des Tieres B innerhalb von 7 Wochen bestätigte diese Ergebnisse. Mittels 16S-rDNA-Sequenzierung wurden die drei Isolate als W. paramesenteroides (Tier A) bzw. W. cibaria (Tier B) identifiziert. Die Analyse durch die Pulsfeldgelelektrophorese (PFGE) ergab ein identisches Smal/Apal-Profil für beide W.-cibaria-Isolate (Kuh B), das sich vom W.-paramesenteroides-Fingerprint der Kuh A unterschied (67% Ähnlichkeit). Diese Untersuchung verweist auf einen möglichen Zusammenhang zwischen dem Nachweis von Weissella spp. und dem Auftreten von Euterentzündungen beim Rind.

Summary

This case report describes the isolation and differentiation of Weissella (W.) spp. from the milk of two cows (A and B) with clinical mastitis (milk changes, asymmetry of the udder and increased somatic cell counts). Quarter milk samples obtained from two dairy cows of different farms had been submitted to the diagnostic laboratory of the Clinic for Ruminants in Vienna for bacteriological examination. Alphahemolytic catalase-negative gram-positive cocci in pure culture on Columbia blood agar were isolated and could not be assigned to a Lancefield group. The isolates were biochemically characterized as Leuconostoc spp. (API® 20 Strep, bioMérieux). A control examination of cow B within 7 weeks confirmed these findings. 16S rDNA sequencing indicated W. paramesenteroides (cow A) and W. cibaria (cow B). The analysis by pulsed-field gel electrophoresis (PFGE) showed identical Smal/Apal profiles for both W. cibaria isolates (cow B), which differed from the W. paramesenteroides fingerprint of cow A (67% similarity). This study indicates a possible relationship between the detection of Weissella spp. and the occurrence of bovine intramammary infections.

Schlüsselwörter

Weissella paramesenteroides - Weissella cibaria - Milchsäurebakterien - Mastitis

Keywords

Weissella paramesenteroides - Weissella cibaria - lactic acid bacteria - mastitis
 

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