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Thromb Haemost 2005; 93(05): 922-926
DOI: 10.1160/TH04-12-0809
Platelets and Blood Cells
Schattauer GmbH

Contrast medium attenuates platelet activation and platelet-leukocyte cross–talk

Qiushang Ji
1   Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden
3   Department of Cardiology, Qilu Hospital of Shandong University, Jinan, China
,
Melania Ghaly
1   Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden
,
Paul Hjemdahl
1   Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden
,
Per Tornvall
2   Cardiology Unit, Karolinska University Hospital (Solna), Stockholm, Sweden
,
Nailin Li
1   Department of Medicine, Clinical Pharmacology Unit, Stockholm, Sweden
› Author AffiliationsGrant support: The study was supported by the grants from the Swedish Heart-Lung Foundation, the Swedish Research Council (5930), China Scholarship Council, the Swedish Medical Association, the Stockholm County Council, and the Karolinska Institute.
Further Information

Publication History

Received 16 December 2004

Accepted after revision 14 February 2005

Publication Date:
11 December 2017 (online)

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Summary

The influence of ionic and non-ionic contrast media (CM) on platelet and leukocyte activation and platelet-leukocyte crosstalk was investigated in hirudinized whole blood. The blood was incubated with and without the ionic CM ioxaglate and the nonionic CM iodixanol at 37ºC for 5 min, without or with stirring. Platelet and leukocyte activation and platelet-leukocyte aggregation were measured using whole blood flow cytometry. When blood samples were pre-incubated in the presence of 2%, 5%, and 10% of CM without stirring, both ioxaglate and iodixanol had little effect on unstimulated samples, but dose-independently decreased 1 μM ADP-induced platelet P-selectin expression and fibrinogen binding, and thus platelet-leukocyte aggregate formation. Ioxaglate had little effect on leukocyte CD11b expression, whilst iodixanol slightly enhanced resting and N-formyl-methionyl-leucyl-phenylalanine (fMLP; 0.1 μM)-stimulated leukocyte CD11b expression. Blood samples were also incubated with stirring to investigate the impact of CM (5% of ioxaglate or iodixanol) on platelet-leukocyte cross-talk. Collagen induced marked platelet activation and platelet-leukocyte aggregation, and subsequently elevated leukocyte CD11b expression. The latter was attenuated by ioxaglate and iodixanol, and was accompanied by reduced platelet-leukocyte aggregation. In conclusion, the CM ioxaglate and iodixanol attenuate platelet activation and platelet-leukocyte cross-talk. Inhibitory effects of the contrast agents on this cross-talk are apparently exerted by reducing heterotypic conjugation, and may be beneficial in connection with PCI.

Keywords

Contrast media - platelets - leukocytes - platelet-leukocyte cross-talk
 

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