Induction of T-Kininogen and Tumor Necrosis Factor-α by Macrophage Migration Inhibitory Factor in vivo

Abstract

Administration of lipopolysaccharide (LPS) induces inflammation and tissue injuries that occasionally results in disseminated intravascular coagulation (DIC). This process is believed to be mediated by vasoactive molecules such as kinins and leads to endothelial damage and obstruction of the microcirculation. In this study, we evaluated the involvement of T-kininogen and macrophage migration inhibitory factor (MIF) in endotoxin-induced systemic inflammation. T-Kininogen is a protein unique to the rat and known as an acute-phase protein in response to endotoxins. Similarly, MIF functions as a proinflammatory cytokine and glucocorticoid-induced immunoregulator. First, we examined the effects of anti-MIF antibody on Wistar King male rats (ca 400 g) treated with intraperitoneal injection of LPS. At 6 hours after LPS injection (5 mg/kg), the platelet counts had decreased from 85 ± 12.8 (× 10⁴/μL) to 8.8 ± 2.6 (× 10⁴/μL). We treated these rats with the anti-rat MIF antibody (5 mg gamma G immunoglobulin [IgG] fraction/kg) 2 hours prior to LPS injection. This treatment prevented the decrease in platelet counts (45.6 ± 5.6 [ X 10>⁴/μL]). Next, we examined the potential of MIF for production of T-kininogen. Intraperitoneal injection of rat MIF significantly upregulated the serum content of T-kininogen at the dose of 500 μg MIF/head. These results imply that MIF and T-kininogen might function in concert in the event of endotoxin-induced inflammation.

Keywords:

Lipopolysaccharide - macrophage migration inhibitory factor - tissue injury - tumor necrosis factor-α