Steroidal Alkaloids from Veratrum japonicum with Genotoxicity on Brain Cell DNA of the Cerebellum and Cerebral Cortex in Mice

Abstract

A new steroidal alkaloid (1), together with three known steroidal alkaloids (2 - 4), has been isolated from the roots and rhizomes of Veratrum japonicum (Baker) Loes. f.., their structures were established as neogermine (1), germine (2), germerine (3), and neogermbudine (4) by means of physicochemical properties and spectroscopic analysis. Compounds 1 - 4 exhibited genotoxicity on brain cells in mice by using single-cell gel electrophoresis (Comet assay).

Key words

Liliaceae - Veratrum japonicum (Baker) Loes. f. - neogermine - genotoxicity

References

• 1 Tang J, Li H L, Huang H Q, Zhang W D. Progress in the research on chemical constituents of Veratrum plants.  Prog Pharm Sci. 2006;  30 206-12.
• 2 Crawford L, Myhr B. A preliminary assessment of the toxic and mutagenic potential of steroidal alkaloids in transgenic mice.  Food Chem Toxicol. 1995;  33 91-4.
• 3 Guo L, Wang L H, Sun B S, Yang J Y, Zhao Y Q, Dong Y X. et al . Direct in vivo evidence of protective effects of grape seed procyanidin fractions and other antioxidants against ethanol-induced oxidative DNA damage in mouse brain cells.  J Agric Food Chem. 2007;  55 5881-91.
• 4 Speit G, Hartmann A. The comet assay (single-cell gel test): a sensitive genotoxicity test for the detection of DNA damage and repair.  Methods Mol Biol. 1999;  113 203-12.
• 5 Sasaki Y F, Saga A, Akasaka M, Nishidate E, Watanabe A M, Ohta T. et al . In vivo genotoxicity of heterocyclic amines detected by a modified alkaline single cell gel electrophoresis assay in a multiple organ study in the mouse.  Mutat. Res1997;  395 57-73.
• 6 Sasaki Y F, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K. et al . The comet assay with 8 mouse organs: results with 39 currently used food additives.  Mutat Res. 2002;  519 103-19.
• 7 Singh N P, Lai H, Khan A. Ethanol-induced single-strand DNA breaks in rat brain cells.  Mutat Res. 1995;  345 191-6.
• 8 Bohlmann F. Lupinen-Alkaloide VIII. Zur Konfigurationsbestimmung von Chinolizidin-Derivaten.  Chem Ber. 1958;  91 2157-67.
• 9 Cong P Z, Li S Y. Natural organic mass spectrometry. In: Cong PZ, editor. Peimine-type alkaloid. Beijing; Chinese Medical Technology Publishing House 2002: 522-5.
• 10 Liang G Y. Overview of the research on alkaloids of Veratrum plants.  J Pharm. 1984;  19 309-20.
• 11 Sayed K AE, Mcchesney J D, Halim A F, Zaghloul A M, Lee I S. A study of alkaloids in Veratrum viride aiton.  Int J Pharm. 1996;  34 161-73.
• 12 Zhao W J, Tezuka Y, Kikuchi T, Chen J, Guo Y T. Studies on the constituents of Veratrum plants II.constituents of Veratrum L. var. ussuriense. (1) structure and ¹H-and ¹³C-nuclear magnetic resonance spectra of a new alkaloid, verussurinine, and related alkaloids.  Chem Pharm Bull. 1991;  39 549-54.