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DOI: 10.1055/s-2007-978546
© Georg Thieme Verlag Stuttgart · New York
Biochemical and Immunochemical Investigations on the Light-Harvesting System of the Cryptophyte Rhodomonas sp.: Evidence for a Photosystem I Specific Antenna
Publication History
1999
1999
Publication Date:
19 April 2007 (online)
Abstract
Thylakoid membranes of the Cryptophyte Rhodomonas sp. were solubilized with the mild detergent dodecyl-β-maltoside and subjected to sucrose density gradient centrifugation. The resulting gradients showed six pigment-bearing bands which were characterized further by means of absorption and fluorescence emission (77 K) spectroscopy, polyacrylamide gel electrophoresis and Western immunoblotting. Two of the bands showed characteristics of light-harvesting complexes, other bands could be attributed to photosystem II and photosystem I. Up to 10 different light-harvesting proteins could be identified, some of which are specific for photosystem I, others for photosystem II. The polypeptides of the light-harvesting complex of photosystem II show a higher chlorophyll c/a ratio than the antenna proteins of photosystem I. As in vascular plants, they represent the bulk of the membrane-intrinsic light-harvesting proteins.
Key words
Rhodomonas - Cryptophyceae - photosynthesis - light-harvesting complexes - photosystems