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Planta Med 1996; 62(5): 423-426
DOI: 10.1055/s-2006-957931
Paper

© Georg Thieme Verlag Stuttgart · New York

A Water-Soluble Antitumor Glycoprotein from Chlorella vulgaris

Kiyoshi Noda1 , Naohito Ohno2 , Kuniaki Tanaka3 , Norikiyo Kamiya3 , Masao Okuda3 , Toshiro Yadomae2 , Kikuo Nomoto4 , Yukihiro Shoyama1
  • 1Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan
  • 2Laboratory of Immunopharmacology of Microbial Products, Tokyo University of Pharmacy and Life Science, School of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-03, Japan
  • 3Research Laboratories, Chlorella Industries Co. Ltd., 1343 Hisatomi, Chikugo City, Fukuoka 833, Japan
  • 4Department of Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan
Further Information

Publication History

1995

1996

Publication Date:
04 January 2007 (online)

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Abstract

An active substance with antitumor activity (ARS2) was purified from the culture media of Chlorella vulgaris and found to be a glycoprotein with a molecular weight of 63,100 amu, as determined by matrix-assisted laser desorption/ ionization (MALDI) mass spectrometry. ARS2 contains 66.9% carbohydrate, mainly D-galactose, and 35.2% protein. The carbohydrate moiety has a β-1,6-D-galactopyranose backbone, as determined by methylation analysis and 13C-NMR. Apparently, the protein moiety, whose 15 amino acid sequence at the NH2-terminus, we determined as DVGEAFPTWDALVA, is necessary for the antitumor activity, as assessed by hydrazinolysis, periodate oxidation, and proteolysis.

Key words

Chlorella vulgaris - Chlorococcales - galactose-rich glycoprotein - methylation analysis - 13C-NMR - antitumor activity

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