Planta Med 2002; 68(5): 463-464
DOI: 10.1055/s-2002-32075
Letter
© Georg Thieme Verlag Stuttgart · New York

Furocoumarins from Angelica dahurica with Hepatoprotective Activity on Tacrine-Induced Cytotoxicity in Hep G2 Cells

Hyuncheol Oh1 , Ho-Sub Lee1 , Taewan Kim2 , Kyu-Yun Chai2 , Hun-Taeg Chung2 , Tae-Oh Kwon2 , Jung-Yang Jun3 , Ok-Sam Jeong3 , Youn-Chul Kim3 , Young-Gab Yun2
  • 1Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Chonbuk, Korea
  • 2Medicinal Resources Research Center, Wonkwang University, Iksan, Chonbuk, Korea
  • 3MRRC and College of Pharmacy, Wonkwang University, Iksan, Chonbuk, Korea
Further Information

Publication History

July 27, 2001

November 10, 2001

Publication Date:
07 June 2002 (online)

Abstract

Fractionation of the MeOH extract of Angelica dahurica Benth et Hook resulted in the isolation of six furocoumarins, imperatorin (1), isoimperatorin (2), (±)-byakangelicol (3), (+)-oxypeucedanin (4), (+)-byakangelicin (5), and (+)-aviprin (6). Among these, compounds 1 and 5 exhibited strong hepatoprotective activities, displaying EC50 values of 36.6 ± 0.98 and 47.9 ± 4.6 μM, respectively. Compounds 3 and 4 showed moderate activities with EC50 values of 112.7 ± 5.35 and 286.7 ± 6.36 μM, respectively. Silybin as a positive control showed the EC50 value with 69.0 ± 3.4 μM. Comparison of hepatoprotective activities for six furocoumarins 1 - 6 suggested that oxy-substitution at the C-9 position increased the hepatoprotective activity.

References

  • 1 Watkins P B, Zimmerman H J, Knapp M J, Gracon S I, Lewis K W. Hepatotoxic effects of tacrine administration in patients with Alzheimer’s disease.  Journal of the American Medical Association. 1994;  271 992-8
  • 2 Grant M H, Duthie S J, Gray A G, Burke M D. Mixed function oxidase and UDP-glucuronyltransferase activities in the human Hep G2 hepatoma cell line.  Biochemical and Pharmacology. 1988;  37 4111-6
  • 3 Viau C J, Curren R D, Wallace K. Cytotoxicity of tacrine and velnacrine metabolites in cultured rat, dog, and human hepatocytes.  Drug & Chemical Toxicology. 1993;  16 227-39
  • 4 Kim C M, Heo M Y, Kim H P, Sin K S, Pachaly P. Pharmacological activities of water extracts of Umbelliferae plants.  Archives of Pharmacal Research. 1991;  14 87-92
  • 5 Dictionary of Natural Products on C D-ROM. Version 8 : 2. London; Chapman & Hall/CRC Press 2000
  • 6 Okamoto T, Yoshida S, Kobayashi T, Okabe S. Inhibition of concanavalin A-induced mice hepatitis by coumarin derivatives.  Japanese Journal of Pharmacology. 2001;  85 95-7
  • 7 Matusuda H, Murakami T, Kageura T, Ninomiya K, Toguchida I, Nishida N. et al . Hepatoprotective and nitric oxide production inhibitory activities of coumarin and polyacetylene constituents from the roots of Angelica furcijuga .  Bioorganic & Medicinal Chemistry Letters. 1998;  8 2191-6
  • 8 Harkar S, Razdan T K, Waight E S. Steroids, chromone and coumarins from Angelica officinalis .  Phytochemistry. 1984;  23 419-26
  • 9 Ishihara K, Fukutake M, Takayuki A, Mizuhara Y, Wakui Y, Yanggisawa T. et al . Simultaneous determination of byak-angelicin and oxypeucedanin hydrate in rat plasma by column-switching high-performance liquid chromatography with ultraviolet detection.  Journal of Chromatography B. 2001;  753 309-14
  • 10 Benoit G G, Naud C F, Simard M A, Astier A L. Noninterference of cytochrome P4501A2 in the cytotoxicity of tacrine using genetically engineered V79 Chinese hamster cells for stable expression of the human or rat isoform and two human hepatocyte cell lines.  Chemical Pharmacology. 1997;  53 423-7
  • 11 Colombain M, Goll V, Muyard F, Girard C, Bévalot F, Richert L. A bioassay using the human hepatoblastoma cell line HepG2 for detecting phototoxicity of furocoumarins.  Planta Medica. 2001;  67 644-6
  • 12 Cho H, Jun J Y, Song E K, Kang K H, Baek H Y, Ko Y S, Kim Y C. Bakuchiol: A hepatoprotective compound of Psoralea corylifolia on tacrine-induced cytotoxicity in Hep G2 cells.  Planta Medica. 2001;  67 750-1

Prof. Young-Gab Yun

Medicinal Resources Research Center

Wonkwang University

Iksan, Chonbuk 570-749, Korea

Email: yunyg@wonkwang.ac.kr

Fax: +82-63-856-5056