Download PDF
Thromb Haemost 1983; 50(04): 838-843
DOI: 10.1055/s-0038-1665324
Original Article
Schattauer GmbH Stuttgart

Platelet Function, Size and Yield in Whole Blood and in Platelet-Rich Plasma Prepared Using Differing Centrifugation Force and Time in Domestic and Food-Producing Animals

R M Clemmons
*   The Department of Medical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
E L Bliss
**   The Department of Preventive Medicine, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
M R Dorsey-Lee
*   The Department of Medical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, WA, U.S.A.
,
C L Seachord
***   The Laboratory of Comparative Thrombosis and Hemostasis, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.
,
K M Meyers
***   The Laboratory of Comparative Thrombosis and Hemostasis, College of Veterinary Medicine, Washington State University, Pullman, WA, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 10 March 1983

Accepted 29 September 1983

Publication Date:
18 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

The effects of centrifugation force and time upon platelets function, mean platelet volume and platelet yield were compared with whole blood platelet counts and size in citrated blood samples from the bovine, canine, caprine, equine, feline, ovine and porcine species. The results were similar, for a given species, irregardless of sample volume. Bovine, caprine, feline and ovine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine, equine and porcine platelet yields and mean platelet volumes were maximal when platelet-rich plasma was prepared using shorter centrifugation times and higher gravitational forces. Platelet aggregation to adenosine diphosphate or arachidonic add was not effected by the method of platelet-rich plasma preparation in bovine, caprine, feline, ovine or pordne platelets. Equine platelet aggregation was maximal when platelet-rich plasma was prepared using longer centrifugation times and lower gravitational forces. Canine platelet aggregation, particularly arachidonic add-induced aggregation, was maximal when platelet-rich plasma was prepared using short centrifugation times and higher gravitational forces. It appeared that the effects of centrifugation parameters upon platelet yield depended upon the relative difference between platelet and red blood cell volumes.

Keywords

Animal species - Platelet-rich plasma preparation - Mean platelet volume - Platelet yield
 

  • References

  • 1 Frojmovic MM, Milton JG. Human platelet size, shape, and related functions in health and disease. Physiol Rev 1982; 62: 185-261
    CrossrefPubMedGoogle Scholar
  • 2 Dodds JW. Second International Registry of Animal Models of Thrombosis and Hemorrhagic Disease. National Academic Press; Washington, D. C: 1981
    Google Scholar
  • 3 Meyers KM, Katz JB, Clemmons RM, Smith JB, Holmsen H. An evaluation of the arachidonate pathway of platelets from companion and food-producing animals, mink, and man. Thromb Res 1980; 20: 13-24
    CrossrefPubMedGoogle Scholar
  • 4 Dodds JW. Platelet function in animals: species specificity. In: Platelets: A Multidisciplinary Approach. de Gaetano G, Garattini G. (eds) Raven Press; New York: 1978. pp 239-258
    Google Scholar
  • 5 Clemmons RM, Meyers KM. Acquisition and aggregation of canine platelets: basic mechanisms of function and differences because of breed origin. J Am Vet Res in press
    Google Scholar
  • 6 Calkins J, Lane KP, Losasso B, Thurber LE. Comparative study of platelet aggregation in various species. J Med 1974; 5: 292-296
    PubMedGoogle Scholar
  • 7 Donner L, Houstkova J. Some properties of blood platelets in animal species. Folia Haematol 1972; 98: 296-302
    PubMedGoogle Scholar
  • 8 Sinakos Z, Caen JP. Platelet aggregation in mammalians. A compara-tive study Thromb Haemostas 1967; 17: 99-111
    PubMedGoogle Scholar
  • 9 Meyers KM, Holmsen H, Seachort CL. Comparative study of dense granule constituents. Am J Physiol 1982; 243: R454-R461
    PubMedGoogle Scholar
  • 10 Prost-Dvojakovic RJ, Le Tohic F, Boulard CH. Study of platelet volumes and diameters in 11 mammals. In: Platelets: Recent Advan-ces in Basic Research and Clinical Aspects. Ulutin ON, Jones JW. (eds) American Elsevier Publishing Co Inc; New York: 1975. pp 30-36
    Google Scholar
  • 11 Ross DW, Ayscue L, Gulley M. Automated platelet counts: accuracy, precision, and range. Am J Clin Pathol 1980; 74: 151-156
    CrossrefPubMedGoogle Scholar
  • 12 Evans VJ, Glasser L. Accuracy of low electronic platelet counts using platelet distribution curves. Am J Med Technol 1981; 47: 15-18
    PubMedGoogle Scholar
  • 13 Bessman JD. Evaluation of automated whole-blood platelet counts and particle sizing. Am J Clin Pathol 1980; 74: 157-162
    CrossrefPubMedGoogle Scholar
  • 14 Heinemann G, Gunner H. Thrombozytenzählung in Vollblut mit einem neuen Zählgerät mit hydrodynamisch fokussierter Kammer. Blut 1980; 40: 275-283
    CrossrefPubMedGoogle Scholar
  • 15 Mehta P, Mehta J, Pepine CJ. Influence of the normal forearm vascular bed on platelet aggregation, counts, and size. Microvas Res 1981; 21: 229-233
    CrossrefPubMedGoogle Scholar
  • 16 Kelton JG, Neame PB, Bishop J, Ali M, Gauldie J, Hirsh J. The direct assay for platelet-associated IgG (PAIgG): lack of association between antibody level and platelet size. Blood 1979; 53: 73-80
    PubMedGoogle Scholar
  • 17 Nelson RB, Kehl D. Electronically determined platelet indices in thrombocytopenic patients. Cancer 1981; 48: 954-956
    CrossrefPubMedGoogle Scholar
  • 18 McLean MR, Hause LL. Collagen-induced platelet aggregation as studied by electronic particle size analysis. Thromb Haemostas 1981; 46: 731-733
    PubMedGoogle Scholar
  • 19 Cortelazzo S, Barbui T, Bassan R, Dini E. Abnormal aggregation and increased size of platelets in myeloproliferative disorders. Thromb Haemostas 1980; 43: 127-130
    PubMedGoogle Scholar
  • 20 Haver VM, Gear AR L. Functional fractionation of platelets. J Lab Clin Med 1981; 97: 187-204
    PubMedGoogle Scholar
  • 21 Karpatkin S. Heterogeneity of rabbit platelets. VI. Further resolution of changes in platelet density, volume, and radioactivity following cohort labelling with 75Se-selenomethione. Br J Haematol 1978; 39: 459-469
    CrossrefPubMedGoogle Scholar
  • 22 Rand ML, Greenberg JP, Packman MA, Mustard JF. Density subpopulations of rabbit platelets: size, protein, and sialic acid content, and specific radioactivity changes following labeling with35S- sulfate in vivo. Blood 1981; 57: 741-746
    PubMedGoogle Scholar