Summary
In order to determine the molecular size of human F VIII as found in fresh plasma
and as found in fresh-frozen plasma and in cryoprecipitate and to study the relationship
of the factor-related properties, namely F VIII:C, F VIIIR:Rcof and F VIIIR:Ag to
F VIII, the factor’s elution pattern on Sephacryl 1000 was investigated. When fresh
plasma was chromatographed on this gel, F VIII eluted in a single sharp peak with
all three F VIII-related activities appearing in the separation range of the gel column.
The molecular weight of F VIII was calculated to be higher than 8 × 105. When fresh-frozen plasma was chromatographed on this gel, the elution pattern was
identical to that of fresh plasma with the single exception that in several samples
F VIII eluted in a broader peak tailing to higher molecular forms. When cryoglobulin
fraction of fresh-frozen plasma was chromatographed, F VIII showed distinct heterogenicity
in the elution pattern with respect to molecular size and relationship to the F VIII-related
properties. Chelation of the endogenous plasma Ca-ions by citrate was found to have
no influence on the factor’s elution pattern.
Keywords
Factor VIII - Factor VIII:C - Factor VIIIR:Ag - Factor VIIIR:Rcof - Molecular weight
