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Thromb Haemost 1979; 42(05): 1598-1603
DOI: 10.1055/s-0038-1657063
Original Article
Schattauer GmbH Stuttgart

Relative Activities of Heparin Fractions Obtained by Gel Chromatography

David T Graham
The National Biological Standards Laboratory, Department of Health, Canberra, A. C. T., Australia
,
Alan R Pomeroy
The National Biological Standards Laboratory, Department of Health, Canberra, A. C. T., Australia
› Author Affiliations
Further Information

Publication History

Received 10 July 1979

Accepted 12 September 1979

Publication Date:
18 February 2019 (online)

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Summary

In order to investigate the reported heterogeneity of commercial heparin injections, lung and mucous preparations were fractionated by gel chromatography according to molecular size. Eluent pools were characterized by measuring electrophoretic mobility, anticoagulant activity using a reactivation assay and an anti-Xa assay, lipolytic activity and the protamine neutralization value. The only biological activity to show a marked relationship with the molecular size of the heparin fraction was the anti-Xa activation activity. This effect was more pronounced with the mucous heparins than with the lung heparin preparation studied.

 

  • References

  • 1 Barrowcliffe TW, Johnson EA, Eggleton CA, Thomas DP. 1977; Anticoagulant activities of lung and mucous heparins. Thrombosis Research 12: 27
    PubMedGoogle Scholar
  • 2 Bianchini P, Guidi G, Osima B. 1972; Blood levels of the clearing factor after administration of a natural glucuronoglucosaminoglycan (3GS). Biochemistry and Experimental Biology. 10: 243
    PubMedGoogle Scholar
  • 3 Denson KW E, Bonnar J. 1973; The measurement of heparin. A method based on the potentiation of anti-factor Xa Thrombosis et Diathesis Haemorrhagica 30: 471
    PubMedGoogle Scholar
  • 4 Hall M, Ricketts CR, Michael SE. 1969; The potency of heparin fractions obtained by gel filtration. Journal of Pharmacy and Pharmacology 21: 626
    CrossrefPubMedGoogle Scholar
  • 5 Johnson EA, Mulloy B. 1976; The molecular-weight range of mucosal-heparin preparations. Carbohydrate Research 51: 1-19
    CrossrefPubMedGoogle Scholar
  • 6 Kavanagh LW, Jaques LB. 1973; Comparison of analytical values for commercial heparins. Arzneimittel-Forschung (Drug Research) 23: 605
    PubMedGoogle Scholar
  • 7 Lane DA, MacGregor IR, Michalski R, Kakkar VV. 1978; Anticoagulant activities of four unfractionated and fractionated heparins. Thrombosis Research 12: 257
    CrossrefPubMedGoogle Scholar
  • 8 Lasker SE, Stivala SS. 1966; Physicochemical studies of fractionated bovine heparin. Archives of Biochemistry and Biophysics 115: 360
    CrossrefPubMedGoogle Scholar
  • 9 Laurent TC. 1961; Studies on fractionated heparin. Archives of Biochemistry and Biophysics 92: 224
    CrossrefPubMedGoogle Scholar
  • 10 Mcduffie NM, Dietrich CP, Nadar HB. 1975; Electrofocusing of heparin: fractionation of heparin into 21 components distinguishable from other acidic mucopolysaccharides. Biopolymers 14: 1473
    CrossrefPubMedGoogle Scholar
  • 11 Sugisaka N, Petracek FJ. 1977; Rapid molecular size determination of heparins by high pressure liquid chromatography. Federation Proceedings 26: 89
    PubMedGoogle Scholar
  • 12 Walton PL, Wright BM. 1964; An automatic coagulometer. Journal of Physiology (London) 172: 7 P
    PubMedGoogle Scholar