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Thromb Haemost 1971; 26(01): 099-102
DOI: 10.1055/s-0038-1653660
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

A Simplified Method for Preparation of Fibrinogen

Louise Walker
1   Department of Materials Science Thornton Hall, University of Virginia School of Engineering and Applied Science Charlottesville, Virginia 22901
,
Avery Catlin
1   Department of Materials Science Thornton Hall, University of Virginia School of Engineering and Applied Science Charlottesville, Virginia 22901
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Publication History

Publication Date:
28 June 2018 (online)

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Summary

A method for preparation of fibrinogen from human plasma is presented. The method, a modification of the glycine precipitation procedure of Kazal (6), is simple and rapid, requiring 8 hours or less. The fibrinogen solution is stable on storage in the refrigerator for at least 7 months, represents an average yield of 30-40% of that in the starting material, and is around 95% clottable. Clottability is good even on storage at 37° C for several days; it is generally around 80% clottable after this storage period. The solution is free of plasminogen and plasmin but contains trace amounts of FSF (fibrin stabilizing factor) and factor VIII (factor VIII activity tends to disappear with storage). Ultracentrifugal analysis shows a main peak comprising approximately 95% of the total area (apparent sedimentation coefficient 5.8 × 10-13) and a smaller peak comprising approximately 5% or less of the total area (apparent sedimentation coefficient 3-3.5 × 10−13).

 

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