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Thromb Haemost 1993; 69(03): 259-261
DOI: 10.1055/s-0038-1651591
Original Article
Fibrinolysis
Schattauer GmbH Stuttgart

The Role of Cyclic Nucleotides in the Release of Tissue-Type Plasminogen Activator and von Willebrand Factor

N Tranquille
The Gaubius Laboratory IVVO-TNO, Leiden, The Netherlands
,
J J Emeis
The Gaubius Laboratory IVVO-TNO, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 07 August 1992

Accepted after revision 13 November 1992

Publication Date:
05 July 2018 (online)

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Summary

The modulation of the induced acute release of tissue-type plasminogen activator (t-PA) and of von Willebrand factor (vWF) by compounds affecting cyclic nucleotide levels was studied, using an isolated rat hindleg perfusion system. Platelet-activating factor (PAF; 5 nM) or bradykinin (0.8 (μM) were used to induce release of t-PA and vWF.

The guanylate cyclase activators sodium nitroprusside and atrial natriuretic factor reduced the induced release of t-PA and vWF. Release was not affected by inhibiting nitric oxide production with NG-nitro-L-arginine. The effects of nitroprusside and atrial natriuretic factor could not be reproduced by infusion of 8-bromo-cGMP.

The adenylate cyclase activator forskolin had no effect on bradykinin-induced release of t-PA and vWF, reduced PAF-induced t-PA release, but potentiated PAF-induced vWF release. These modulatory effects were only partially mimicked by infusion of 8-bromo-cAMP.

None of the compounds tested was able to induce the release of t-PA or of vWF in the absence of stimulation by bradykinin or platelet-activating factor. Cyclic nucleotides can thus modulate, but not induce, the acute release of t-PA and vWF from perfused rat hindlegs.

 

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