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Thromb Haemost 1996; 75(02): 326-331
DOI: 10.1055/s-0038-1650269
Original Article
Schattauer GmbH Stuttgart

Aggregated, Conformationally Changed Fibrinogen Exposes the Stimulating Sites for t-PA-Catalysed Plasminogen Activation

Unni Haddeland
1   The Research Institute for Internal Medicine, Rikshospitalet, The Netherlands
,
Knut Sletten
2   Department of Biochemistry/Biotechnology Centre of Oslo, University of Oslo, Oslo, Norway
,
Anne Bennick
1   The Research Institute for Internal Medicine, Rikshospitalet, The Netherlands
,
Willem Nieuwenhuizen
3   Gaubius Institute TNO, Leiden, The Netherlands
,
Frank Brosstad
1   The Research Institute for Internal Medicine, Rikshospitalet, The Netherlands
› Author Affiliations
Further Information

Publication History

Received: 09 June 1995

Accepted after revision03 November 1995

Publication Date:
26 July 2018 (online)

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Summary

The present paper shows that conformationally changed fibrinogen can expose the sites Aα-(148-160) and γ-(312-324) involved in stimulation of the tissue-type plasminogen activator (t-PA)-catalysed plasminogen activation. The exposure of the stimulating sites was determined by ELISA using mABs directed to these sites, and was shown to coincide with stimulation of t-PA-catalysed plasminogen activation as assessed in an assay using a chromogenic substrate for plasmin. Gel permeation chromatography of fibrinogen conformationally changed by heat (46.5° C for 25 min) demonstrated the presence of both aggregated and monomeric fibrinogen. The aggregated fibrinogen, but not the monomeric fibrinogen, had exposed the epitopes Aα-(148-160) and γ-(312-324) involved in t-PA-stimulation. Fibrinogen subjected to heat in the presence of 3 mM of the tetrapeptide GPRP neither aggregates nor exposes the rate-enhancing sites. Thus, aggregation and exposure of t-PA-stimulating sites in fibrinogen seem to be related phenomena, and it is tempting to believe that the exposure of stimulating sites is a consequence of the conformational changes that occur during aggregation, or self-association. Fibrin monomers kept in a monomeric state by a final GPRP concentration of 3 mM do not expose the epitopes Aα-(148-160) and γ-(312-324) involved in t-PA-stimulation, whereas dilution of GPRP to a concentration that is no longer anti-polymerizing, results in exposure of these sites. Consequently, the exposure of t-PA-stimulating sites in fibrin as well is due to the conformational changes that occur during selfassociation.

 

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