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Thromb Haemost 1973; 30(01): 229-234
DOI: 10.1055/s-0038-1649069
Original Article
Schattauer GmbH

Preparation of Modified Bovine Factor VIII with Enhanced Biological Activity Using Insoluble-trypsin Columns

Christine N. Vogel
1   Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)
,
Henry E. Parfitt Jr.
1   Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)
,
Henry S. Kingdon
1   Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)
,
Roger L. Lundblad
1   Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)
› Author Affiliations
Further Information

Publication History

Received for publication 16 May 1973

Accepted for publication 03 August 1973

Publication Date:
30 June 2018 (online)

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Summary

Crude bovine Factor VIII preparations have been modified by passage over a column of trypsin-agarose. Factor VIII prepared without an early adsorption step utilizing either barium sulfate or aluminum hydroxide gel generated significant Factor Xa activity by passage over such a column and was unsuitable for further characterization. Factor VIII prepared using adsorption with barium sulfate contained no Factor Xa activity after passage over trypsin-agarose. This trypsin-modified material showed a decreased molecular size as judged by behavior on gel filtration and an increased rate of reaction with bovine Factor IXa.

 

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