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Thromb Haemost 1976; 35(01): 167-177
DOI: 10.1055/s-0038-1647941
Original Article
Schattauer GmbH

Comparison of Two Types of Endothelial Cells in Long Term Culture[*]

Bonnie Joy Sedlak   **
1   Department of Biochemistry, Rush University, Chicago, Ill. 60612, USA
,
F. M Booyse
1   Department of Biochemistry, Rush University, Chicago, Ill. 60612, USA
,
S Bell
1   Department of Biochemistry, Rush University, Chicago, Ill. 60612, USA
,
M. E Rafelson Jr.
1   Department of Biochemistry, Rush University, Chicago, Ill. 60612, USA
› Author Affiliations
Further Information

Publication History

Received 06 August 1975

accepted 06 August 1975

Publication Date:
02 July 2018 (online)

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Summary

Bovine aortic endothelial cells have been serially subcultured and maintained for ten months (24 passages) without any apparent loss of endothelial-like properties. There appear to be two endothelial cell types; one type is mononucleated, 40—60 μ in diameter, and the other (giant) cell type, multinucleate and 300-600 μ in diameter. Both cell types were identified as endothelial cells by established criteria; 1. ultrastructurally, by the presence of Weibel-Palade bodies, microfilaments and pinocytotic vesicles and 2. immunologically, by the presence of factor VIII antigen and thrombosthenin.

* Presented at the Vth Congress of the International Society on Thrombosis and Haemostasis, Paris, July, 1975.


** Present address: The Department of Biological Sciences, Smith College, Northampton, Mass. 01060.


 

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