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Thromb Haemost 1979; 41(02): 392-406
DOI: 10.1055/s-0038-1646788
Original Articles
Schattauer GmbH Stuttgart

Complement-Dependent and Complement-Independent Interactions of Bacterial Lipopolysaccharides and Mucopeptides with Rabbit and Human Platelets

N Semeraro
*   On leave of absence from the Department of Microbiology (Head: Prof. D. Fumarola), Medical School, University of Bari, Bari, Italy
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
,
M Colucci
*   On leave of absence from the Department of Microbiology (Head: Prof. D. Fumarola), Medical School, University of Bari, Bari, Italy
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
,
J Vermylen
The Center for Thrombosis and Vascular Research, Department of Medical Research, University of Leuven, Belgium
› Author Affiliations
Further Information

Publication History

Received 10 January 1978

Accepted 15 March 1978

Publication Date:
09 July 2018 (online)

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Summary

The effect of 10 commercial lipopolysaccharides (LPS) and of 5 highly purified LPS with variable but defined polysaccharide content, two LPS from anaerobic bacteria, two mucopeptides and two meningococcal polysaccharides, was studied on rabbit and human platelets. All the LPS preparations induced aggregation in rabbit heparinized platelet-rich plasma (PRP) but to differing degrees. However, a preparation consisting essentially of lipid A (from Salmonella minnesota Re 595) was one of the most active. The mucopeptides were very potent whereas the meningococcal polysaccharides had no effect. The activity was abolished by inactivation of complement. The lack of ability of LPS and mucopeptides to aggregate rabbit platelets in ethyleneglycol tetraacetic acid (EGTA) – PRP suggests that the mechanism depends on activation of the classical pathway of complement. None of the bacterial products induced aggregation of human platelets.

When washed rabbit platelets are mixed with complement-depleted rabbit serum and calcium chloride, generation of thrombin occurs. Washed platelets contribute to thrombin generation by providing factor V, a factor X activating activity, and possibly phospholipid (Brit. J. Haemat. 36: 107, 1977). All the LPS preparations but not the mucopeptides or meningococcal polysaccharides enhanced the rate of thrombin formation by enhancing the factor X activating activity of rabbit or human platelets.

It is concluded that LPS affect rabbit platelets both by complement-dependent and complement-independent mechanisms, but human platelets only by the complement- independent pathway. Mucopeptides react with platelets only by the complement-dependent way and have no effect on human platelets.

 

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