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Thromb Haemost 1999; 82(01): 145-148
DOI: 10.1055/s-0037-1614644
Scientific and Standardization Committee Communication
Schattauer GmbH

A Soluble Fibrin Standard: Comparable Dose-response with Immunologic and Functional Assays[*]

Bonnie I. McCarron
1   From the Vascular Medicine Unit, Department of Medicine University of Rochester School of Medicine and Dentistry Rochester, New York, USA
,
Victor J. Marder
1   From the Vascular Medicine Unit, Department of Medicine University of Rochester School of Medicine and Dentistry Rochester, New York, USA
,
Joel J. Kanouse
1   From the Vascular Medicine Unit, Department of Medicine University of Rochester School of Medicine and Dentistry Rochester, New York, USA
,
Charles W. Francis
1   From the Vascular Medicine Unit, Department of Medicine University of Rochester School of Medicine and Dentistry Rochester, New York, USA
› Author AffiliationsThis work was supported in part by Grants HL-07152 and HL-30616 from the National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland.
Further Information

Publication History

Received 14 January 1998

Accepted after resubmission 19 February 1999

Publication Date:
11 December 2017 (online)

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Summary

A soluble fibrin (SF) preparation has been developed as a potential standard by the Scientific and Standardization Committee for use in assays evaluating in vitro preparations and patient plasma samples. The SF standard was prepared by reaction of factor XIII-free fibrinogen with thrombin, followed by neutralization with hirudin and solubilization of the fibrin in acetic acid. As characterized by SDS-PAGE, the polypeptide chain structure shows the anticipated loss of fibrinopep-tides and lack of sγ or α chain crosslinking. The standard was added to pooled normal plasma at concentrations from 12.5 μg/ml to 340 μg/ml and tested with four commercially available assays based on immunologic reactions using ELISA or latex agglutination or on t-PA cofactor activity for plasminogen to plasmin conversion. Absolute “soluble fibrin” concentrations were calculated using the manufacturers’ calibrators and showed distinct dose-response relationships for each assay. Expression of the results following log-transformation produced a series of parallel lines, indicating that this SF preparation can serve as a standard, effectively normalizing the disparate proprietary internal calibrators currently used for each assay.

* Report prepared on behalf of the DIC Subcommittee of the SSC


 

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