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Drug Res (Stuttg) 2016; 66(04): 203-210
DOI: 10.1055/s-0035-1564137
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Pharmacological Evaluation of Central Nervous System Effects of Ethanol Leaf Extract of Olax Subscorpioidea in Experimental Animals

O. A. Adeoluwa
1   Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
2   Department of Pharmacology, College of Medicine and Health Sciences, Afe Babalola University Ado-Ekiti, Ekiti State, Nigeria
,
A. O. Aderibigbe
1   Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
,
G. O. Agu
1   Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria
› Author Affiliations
Further Information

Publication History

received 04 February 2015

accepted 20 August 2015

Publication Date:
01 December 2015 (online)

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Abstract

Background: Olax subscorpioidea Oliv. (Olacaceae) is a medicinal plant used in folk medicine in the management of pain, mental illness, and convulsion. We evaluated neurosedative and anticonvulsant properties of the ethanol leaf extract of O. subscorpioidea (ELEOS).

Methods: Effects of ELEOS (3.125, 6.25, 12.5, 25 mg/kg) on novelty-induced behaviors were determined using open field test. Anxiolytic effect of ELEOS (3.125, 6.25, 12.5, 25 mg/kg) was assessed using hole-board and elevated-plus maze paradigms. The effect of O. subscorpioidea on pentobarbitone sleeping time was also investigated. Anticonvulsant property of ELEOS (12.5–50 mg/kg) was evaluated using pentylenetetrazole, picrotoxin and strychnine-induced convulsions assays. The extract was administered once intraperitoneally.

Results: The LD50 of ELEOS was 300 mg/kg. ELEOS (3.125, 6.25, 12.5, 25 mg/kg) significantly reduced rearing (99.8±2.8, 76.2±2.9, 37.4±1.2, 5.8±0.8) and grooming (48.0±3.6, 33.8±2.9, 25.4±1.6, 7.6±0.8) as compared with controls (185.8±5.1; 63.8±4.3). Treatment with ELEOS (3.125, 6.25, 12.5, 25 mg/kg) significantly reduced head-dipping on hole-board (10.6±1.9, 8.8±1.2, 7.2±0.9, 6.0±1.1) as compared with control (27.8±1.5). However, there was no anxiolytic effect on EPM. ELEOS (12.5, 25, 50 mg/kg) significantly prolonged pentobarbitone-induced sleeping time (43.0±1.4, 51.0±1.2, 61.0±1.8) as compared with control (31.0±0.7). At 50 mg/kg, ELEOS significantly prolonged onset of seizure (2.72±2.07) and latency to death (9.20±1.24) as compared with controls (0.54±0.02; 2.00±0.44) in pentylenetetrazole-induced convulsions with no effect on picrotoxin and strychnine-induced convulsions.

Conclusion: The ELEOS is sedative and has mild anticonvulsant activity and this study supports pharmacological basis for its use in the management of mental illness and convulsion.

Key words

convulsion - neurobehavioral - pentylenetetrazole - pentobarbitone
 

  • References

  • 1 Bouquet A. Féticheurs et médecines traditionnelles du Congo (Brazzaville). Mém. O.R.S.T.O.M. 1969 36. 282 http://www.docstoc.com/docs/41737230/Ficheurs-et-mecines-traditionn
    PubMedGoogle Scholar
  • 2 Sonibare A, Gbile ZO Ethnobotanical survey of anti-asthmatic plants in south western Nigeria. Afr J Trad CAM 2008; 5: 340-345
    PubMedGoogle Scholar
  • 3 Mike OS, Amusa NA, Raji-Esan SO et al. Ethnobotanical Survey of Anti-Cancer Plants in Ogun State, Nigeria. Annals of Biological Research 2010; 1: 261-273
    PubMedGoogle Scholar
  • 4 Ibrahim JA, Muazzam I, Jegede IA et al. Ethno-medicinal plants and methods used by Gwandara tribe of Sabo Wuse in Niger state, Nigeria, to treat mental illness. African Journal of Traditional, Complementary and Alternative Medicines 2007; 4: 211-218
    PubMedGoogle Scholar
  • 5 Adjanohoun EV, Adjakidje MRA, Ahyi K et al. Contribution aux études ethnobotaniques et floristiques au Togo. Agence de coopération culturelle et technique, (A.C.C.T.), Paris, 671 p. From the data bank PHARMEL 2 (ref. HP 10) 1986
    PubMedGoogle Scholar
  • 6 Ayandele AA, Adebiyi AO. The phytochemical analysis and antimicrobial screening of extracts of olax subscorpioidea. African Journal of Biotechnology 2007; 6: 868-870
    PubMedGoogle Scholar
  • 7 Ukwe CV, Ubaka CM, Madusque UJ. Evaluation of the antiulcer activity of Olax subscorpioidea Oliv. roots in rats. Asian Pacific Journal of Tropical Medicine 2010; 3: 13-16
    CrossrefPubMedGoogle Scholar
  • 8 Adeoluwa OA, Aderibigbe AO, Bakre AG. Evaluation of antidepressant-like effect of Olax subscorpioidea Oliv. (Olacaceae) extract in mice. Drug Research 2015; 65: 306-311
    Article in Thieme ConnectPubMedGoogle Scholar
  • 9 Adeoluwa OA, Aderibigbe AO, Olonade ET. Antinociceptive property of ethanol leaf extract of Olax subscorpioidea. Journal of Ethnopharmacology 2014; 156: 353-357
    CrossrefPubMedGoogle Scholar
  • 10 Lorke D. A new approach to practical acute toxicity testing. Arch Technol 1983; 54: 275-282
    PubMedGoogle Scholar
  • 11 Ajayi AA, Ukponmwan OE. Evidence of Angiotensin II and Endogenous opioid modulation of NIR in the Rat. African Journal of Medicine & Medical Science 1994; 23: 287-290
    PubMedGoogle Scholar
  • 12 Onigbogi O, Ajayi AA, Ukponmwan OE. Mechanisms of Chloroquine- Induced Body Scratching Behaviour in Rats: Evidence of involvement of endogenous opioid peptides. Pharmacology Biochemistry and Behaviour 2000; 65: 333-337
    CrossrefPubMedGoogle Scholar
  • 13 Aderibigbe AO, Agboola OI. Neuropharmacological profile of Struchium sparganophora (Linn) O. Ktze in mice. Asian Journal of Traditional Medicines 2011; 6: 3
    PubMedGoogle Scholar
  • 14 Akanmu MA, Olowookere TA, Atunwa SA et al. Neuropharmacological effects of nigerian honey in mice. African Journal of Traditional Complementary Alternative Medicine 2011; 8: 230-249
    PubMedGoogle Scholar
  • 15 Dorr M, Joycee D, Porsolt RD et al. Persistence of dose-related behaviour in mice. Nature 1971; 231: 121-123
    CrossrefPubMedGoogle Scholar
  • 16 Lister RG. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology (Berl) 1987; 92: 180-185
    PubMedGoogle Scholar
  • 17 Ferrini R, Miragoli G, Taccardi B. Neuro-pharmacological studies on SB 5833, a new psychotherapeutic agent of the benzodiazepine class. Arzneim.-Forsch. (Drug Res) 1974; 24: 2029-2032
    PubMedGoogle Scholar
  • 18 Hosseinzadeh H, Parvardeh S. Anticonvulsant effects of thymoquinone, the major constituent of Nigella sativa seeds in mice. Phytomedicine 2004; 11: 56-64
    CrossrefPubMedGoogle Scholar
  • 19 Labella FS, Punsky C, Havlicek V. Morphine derivatives with diminished opiate receptor potency show enhanced control excitatory activity. Brain Research 1979; 174: 263-271
    CrossrefPubMedGoogle Scholar
  • 20 Blanchard DC, Griebel G, Blanchard RJ. Mouse defensive behaviors: Pharmacological and behavioral assays for anxiety and panic. Neuroscience and Biobehavioral Reviews 2001; 25: 205-218
    CrossrefPubMedGoogle Scholar
  • 21 Hellion-Ibarrola MC, Ibarrola DA, Montalbetti I et al. Acute toxicity and general pharmacological effect on central nervous system of the crude rhizome extract of Kyllinga brevifolia Rottb. Journal of Ethnopharmacology 1999; 66: 271-276
    CrossrefPubMedGoogle Scholar
  • 22 Akanmu MA, Adeosu SO, Ilesanmi OR. Neuropharmacological effects of oleaminde in male and female mice. Behavioural Brain Research 2007; 182: 88-94
    CrossrefPubMedGoogle Scholar
  • 23 Spruijt BM, VanHooff JARAM, Gispen WH. Ethology and neurobiology of grooming behavior. Physiol Rev 1992; 72: 825-852
    PubMedGoogle Scholar
  • 24 Ukponmwan OE, Poel-Heisterkamp L, Dzoljic MR. REM sleep deprivation decreases grooming and scratching behavior induced by enkephalinase inhibition or opiate withdrawal. Pharmacology Biochemistry and Behaviour 1985; 23: 385-389
    CrossrefPubMedGoogle Scholar
  • 25 Gispen WH, Isaacson RL. ACTH-induced excessive grooming in the rat. Pharmacol Ther 1981; 12: 209-246
    CrossrefPubMedGoogle Scholar
  • 26 Walting Keith J. Overview of central nervous system receptors. In: Keith J. Walting. (Eds.) The RBI Handbook of Receptor Clarification and signal Transduction. 3rd ed. RBI; Ntick, MA: 1998: 2-45
    Google Scholar
  • 27 Griebel G, Moreau JL, Jenck F et al. Acute and chronic treatment with 5-TH reuptake inhibitors differentially modulate emotional responses in anxiety models in rodents. Psychopharmacology 1994; 113: 463-470
    CrossrefPubMedGoogle Scholar
  • 28 Beninger RJ. The role of dopamine in locomotor activity and learning. Brain Research Reviews 1983; 6: 173-196
    CrossrefPubMedGoogle Scholar
  • 29 Dulawa SC, Grandy DK, Low MJ et al. Dopamine D4 receptor-knock-out mice exhibit reduced exploration of novel stimuli. J Neurosci 1999; 19: 9550-9556
    PubMedGoogle Scholar
  • 30 Carlsson A, Lindquist M, Magnusson T et al. On the presence of 3 hydroxytyramine in brain. Science 1958; 129: 471-473
    PubMedGoogle Scholar
  • 31 Fray PJ, Sahakian BJ, Robbins TW et al. An observational method for quantifying the behavioural effects of dopamine agonists: Contrasting effects of D-amphetamine and apomorphine. Psychopharmacology 1980; 69: 253-259
    CrossrefPubMedGoogle Scholar
  • 32 File SE, Wardill AG. Validity of head dipping as a measure of exploration in a modified hole-board. Psychopharmacologia 1975; 44: 53-59
    CrossrefPubMedGoogle Scholar
  • 33 Crawley JN. Exploratory behaviour models of anxiety in mice. Neuroscience and Behavioural Review 1985; 9: 37-44
    PubMedGoogle Scholar
  • 34 Bilkei-Gorzo A, Gyertyan I. Some doubts about the basic concept of hole board test. Neurobiology (Bp) 1996; 4: 405-415
    PubMedGoogle Scholar
  • 35 Sayin U, Cengiz S, Altug T. Vigabatin as an anticonvulsant against penteletetrazole seizures. Pharmacological Research 1993; 28: 325-331
    CrossrefPubMedGoogle Scholar
  • 36 Loscher W, Frey HH. Naunyn-Schnmiedeberg’s Arch. Pharmacol 1977; 296: 263
    PubMedGoogle Scholar
  • 37 Abdul-Ghani ΑS, El-Lati SG, Saca’an AI et al. Int J Crude. Drug Res 1987; 25: 39
    PubMed
  • 38 Rang HP, Dale MM, Ritter JM et al. Pharmacology. 5th ed. India: Churchill Livingstone; 2005: 456-473
    Google Scholar
  • 39 De Sarro A, Cechetti V, Fravolini V et al. Effects of novel 6-desfluroquinolones and classic quinolones on Pentylenetetrazole-induced seizures in mice. Antimicrobial Agents Chemotherapy 1999; 43: 1729-1736
    PubMedGoogle Scholar
  • 40 Gale K. Subcortical structures and pathways involved in convulsive seizures generation. Journal of Clinical Neurophysiology 1992; 2: 264-277
    PubMedGoogle Scholar
  • 41 Westmoreland BF, Benarroch EE, Dube JR et al. Medical neurosciences. Mayo Foundation Rochester 1994; 307-312
    PubMedGoogle Scholar
  • 42 Corda MG, Costa E, Guidtti A. Specific pro-convulsant action of an imidazobendiate pine (RO-15-1788) on isoniazid convulsions. Neuropharmacology 1982; 21: 91-94
    CrossrefPubMedGoogle Scholar
  • 43 Kendall DA, Fox DA, Enna SJ. Anticonvulsant profile of gamma vinyl GABA. Neuropharmacology 1981; 20: 4-10
    PubMedGoogle Scholar
  • 44 Coulter DA, Hugenard JR, Prince DA. Characterization of the ethosuximide reduction of low-threshold calcium current in thalamic neurons. Ann Neurol 1989; 25: 582-593
    CrossrefPubMedGoogle Scholar
  • 45 Davies JA. Mechanisms of action of antiepileptic drugs. Seizure 1995; 4: 267-271
    CrossrefPubMedGoogle Scholar
  • 46 Adjanohoun EV, Adjakidje MRA, Ahyi K et al. 1986 Contribution to ethnobotanical and floristic studies in Togo. Agency for Cultural and Technical Cooperation, (A.C.C.T.), Paris, 671 p. From the data bank PHARMEL 2 (ref. HP 10)
    PubMedGoogle Scholar
  • 47 Du XM, Sun NY, Takizawa N et al. Sedative and anticonvulsant activities of goodyerin, a flavonol glycoside from Goodyera schlechtendaliana. Phytother Res 2002; 16: 261-263
    CrossrefPubMedGoogle Scholar
  • 48 Griebel G, Perrault G, Tan S et al. Pharmacological studies on synthetic flavonoids: comparision with diazepam. Neuropharmacol 1999; 38: 965-977
    CrossrefPubMedGoogle Scholar
  • 49 Aguirre-Hernandez E, Gonzalez-Trujano ME, Martinez AL et al. HPLC/MS and anxiolytic-like effect of quercetin and kaemferol flavonoids from Tilia Americana var. maxicana . Journal of Ethnopharmacology 2010; 127: 91-97
    CrossrefPubMedGoogle Scholar
  • 50 Salgueiro JB, Ardenghi P, Dias M et al. Anxiolytic natural and synthetic flavonoid ligands of the central benzodiazepine receptor have no effect on memory tasks in rats. Pharmacol Biochem Behav 1997; 58: 887-891
    CrossrefPubMedGoogle Scholar