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Drug Res (Stuttg) 2013; 63(06): 300-304
DOI: 10.1055/s-0033-1337939
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

A Validated and Stability Indicating HPLC Method for Analysis of Diminazene Aceturate and Antipyrine Combination in a Ready Injectable Solution

M. N. Abualhasan
1   Department of Pharmacy, An-Najah National University, Nablus, Palestine
,
N. Batrawi
2   Advanced Veterinary Company, Ramallah, Palestine
,
A. N. Zaid
1   Department of Pharmacy, An-Najah National University, Nablus, Palestine
,
D. G. Watson
3   Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK
› Author Affiliations
Further Information

Publication History

received 21 December 2012

accepted 17 February 2013

Publication Date:
26 March 2013 (online)

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Abstract

Diminazene aceturate and Antipyrine combination therapy is widely used in veterinary medicine. A simple reverse HPLC method for the analysis of samples of a ready injectable formulation containing a mixture of active ingredients and inactive excipients has been developed. The HPLC analysis was carried out using a reversed phase (RP)-C18 (250 mm×4.0 mm, 5 μm) column. The isocratic mobile phase consisted of a mixture of acetonitrile, methanol, phosphate buffer and hexane sulfonate; the flow rate was 0.6 mL/min and ultraviolet detection was at 291 nm. This method was validated in accordance with FDA and ICH guidelines and showed good linearity, accuracy, precision, selectivity and the system suitability results were within the acceptance criteria. A stability-indicating study was also carried out and indicated that this method could be used for purity and degradation evaluation of these formulations.

Key words

HPLC - diminazene - antipyrine - stability-indicating
 

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