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Planta Med 2011; 77(10): 1054-1059
DOI: 10.1055/s-0030-1270740
Biological Screening
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Screening and Bioguided Fractionation of Amaryllidaceae Species with Anti-Trichomonas vaginalis Activity

Patrícia de Brum Vieira1 , Raquel Brandt Giordani2 , Geraldo Attilio De Carli3 , José Angelo Silveira Zuanazzi2 , Tiana Tasca1
  • 1Laboratório de Pesquisa em Parasitologia, Departamento de Análises, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
  • 2Laboratório de Farmacognosia, Departamento de Produção de Matéria Prima, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
  • 3Instituto de Geriatria e Gerontologia, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brasil
Further Information

Publication History

received October 10, 2010 revised January 3, 2011

accepted January 15, 2011

Publication Date:
09 February 2011 (online)

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Abstract

The Amaryllidaceae family is known by its ornamental and medicinal value and has attracted considerable attention due to the content of alkaloids of its species, which showed interesting biological properties. The present study evaluated the anti-Trichomonas vaginalis activity of eighteen extracts (12.5 to 0.19 mg/mL) and six isolated alkaloids (125 to 1.9 µg/mL) from Amaryllidaceae species. The alkaloids diminished the trophozoites viability (from 15 to 40 %). The extracts from Hippeastrum breviflorum demonstrated the highest anti-T. vaginalis activity (viability was 60 % reduced), and a bioguided study was conducted. Six fractions with antiprotozoal activity had lycorine and lycosinine as major components suggesting a synergistic effect, taking into account the higher anti-T. vaginalis activity of extracts when compared to isolated alkaloids. Our results point out the antiprotozoal potential of the Amaryllidaceae species against T. vaginalis. This parasite causes trichomonosis, the most prevalent nonviral sexually transmitted diseases (STD) worldwide and a public health problem that requires new therapeutic alternatives as well bioactive natural products.

Key words

Trichomonas vaginalis - anti‐Trichomonas activity - Amaryllidaceae - Hippeastrum

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Prof. Dra. Tiana Tasca

Laboratório de Pesquisa em Parasitologia, Departamento de Análises
Faculdade de Farmácia
Universidade Federal do Rio Grande do Sul

Av. Ipiranga 2752

90610-000 Porto Alegre, RS

Brasil

Phone: +55 51 33 08 53 25

Fax: +55 51 33 08 54 37

Email: tiana.tasca@farmacia.ufrgs.br

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