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Exp Clin Endocrinol Diabetes 1990; 96(5): 129-137
DOI: 10.1055/s-0029-1211001
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Radioimmunoassay of Corticotropin - Releasing Hormone

Yvonne Rückert, W. Rohde, J. Furkert
  • Institute of Experimental Endocrinology (Head: Prof. Dr. se. med. Dr. h. c. G. Dörner),
    Department of Internal Medicine (Head: Prof. Dr. med. habil. H. Berndt), Humboldt University Medical School (Charité) and Institute of Drug Research (Head: Prof. Dr. med. habil. P. Oehme), Academy of Sciences of the GDR, Berlin/GDR
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Publication History

1990

Publication Date:
16 July 2009 (online)

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Summary

A specific double antibody radioimmunoassay (RIA) for human-Corticotropin-Releasing Hormone (hCRF) using an antibody to synthetic hCRF was established. This antibody allowed a usable range of 10 pg to 5 ng of CRF per ml in the assay.

Comparing the efficiency of various plasma extraction procedures, the extraction with ice-cold methanol was found to be the most simple and rapid method with an extraction efficiency of more than 80%.

The reliability of the radioimmunoassay was shown under physiological and pathophysiological conditions.

Key words

CRF - Radioimmunoassay - Plasma extraction - Normal and pathological values

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