PDF herunterladen
Exp Clin Endocrinol Diabetes 1984; 84(4): 63-70
DOI: 10.1055/s-0029-1210367
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

A Sensitive Direct Enzyme Immunoassay for Cortisol in Plasma and Saliva1)

W. Hubl, H. Taubert, E. Freymann, D. Meissner, F. Stahl, G. Dörner
  • Institute of Clinical Chemistry and Laboratory Diagnosis (Head: Dr. rer. nat. D. Meißner) of Dresden-Friedrichstadt-Hospital (Medical Director: OMR Prof. Dr. sc. med O. Günther), VEB Sächsisches Serumwerk Dresden (Director: Obering. M. Kluge), Institute of Experimental Endocrinology of Humboldt University (Charité) (Director: Prof. Dr. sc. med. G. Dörner) Berlin/GDR
1) Supported by the research project of Medizinische Laboratoriumsdiagnostik of Ministry of Health/GDR
Weitere Informationen

Publikationsverlauf

1983

Publikationsdatum:
17. Juli 2009 (online)

Auch verfügbar auf
  als PDF herunterladen  Lizenzen und Reprints

Summary

A sensitive enzyme immunoassay for cortisol in plasma and saliva without extraction has been developed. An antibody was raised in rabbits against cortisol-3-CMO-BSA and compared with anti-cortisol-21-HS-HSA. The enzyme label was a covalently linked cortisol-horseradish peroxidase conjugate. Solvent extraction was avoided by use of heat denaturation of transcortin and dilution of plasma 1:300. This assay had a limit of detection of 3 fmol/tube and satisfied the standard criteria of accuracy and precision. The test requires only a simple photometer.

The normal values of cortisol in plasma and saliva before and after ACTH, dexamethasone or metyrapone application were determined. Saliva, being easily collected by a noninvasive, stress-free technique is an alternative to plasma. The cortisol concentrations were significantly increased in plasma and saliva of patients with Cushing's syndrome and decreased in patients with Addison's disease.

Key words

Enzyme immunoassay - Saliva - Cortisol - Cushing syndrome - Addison disease

      >