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DOI: 10.1055/s-0028-1112114
© Georg Thieme Verlag KG Stuttgart · New York
Time Course of Effects of Inhalation Anesthesia on Blood Glucose Level in Male and Female C57BL/6 Mice
Publication History
received 13.08.2008
accepted 17.11.2008
Publication Date:
15 January 2009 (online)
Introduction
Mice are the most common animal model used for in vivo studies relevant to human disease. The numerous mouse phenotypes available, created by genetic manipulation, enable the linking of specific genes to normal or abnormal molecular and organ functions. Isoflurane is a very common type of anesthesia used in many laboratories performing metabolism and diabetes research. The study of diabetes and obesity focuses on the glucose level as one of the main variables. Moreover, microPET imaging techniques specifically focus on blood glucose biodistribution [1] [2] [3] [4]. The handling and preparation of mice (stress, body temperature, and anesthesia) are very important factors that can impact on glucose levels. Pomplun et al. [5] have previously reported that blood glucose levels in wild type C57Bl/6 mice were significantly elevated immediately following isoflurane anesthesia while Fueger et al. [6] reported that isoflurane anesthesia (2% in 100% oxygen) mildly increased blood glucose levels in severe combined immunodeficient (SCID) male mice [5] [6]. Other investigators have reported development of hypoglycemia during isoflurane anesthesia [1] [2] [7].
Since we routinely use isoflurane, a common type of inhalation anesthesia, in our imaging studies we examined its impact on blood glucose levels in male and female mice. Mice exhibited an elevation in blood glucose levels immediately after the period of anesthesia. This effect was significant for male mice. Subsequently, glucose readings declined significantly, and eventually attained a reading very similar to the initial reading, pre-anesthesia.
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Correspondence
J. L. Durand
Department of Physiology & Biophysics
Albert Einstein College of Medicine
Bronx
10461 New York
USA
Phone: 778/430 32 57/819
Fax: 778/430 32 57/819
Email: jdurand@aecom.yu.edu