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Horm Metab Res 1970; 2(6): 330-332
DOI: 10.1055/s-0028-1095048
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© Georg Thieme Verlag KG Stuttgart · New York

Lipogenic Activity of a Potent Lipolytic Hormone: Sheep β Lipotropin (β-LPH)

A Comparative Study of the Effects of β-LPH and Insulin on In Vitro Glucose Metabolism of Adipose TissueA.  Gattereau , M.  Chrétien , P.  Bielmann
  • Nutrition and Pituitary Hormones Laboratories, Clinical Research Institute, Montreal, P.Q., Canada
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Publication Date:
08 January 2009 (online)

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Abstract

The objective of the studies reported here was to investigate the effect of sheep β-LPH in the presence or absence of insulin on glucose utilization by rat adipose tissue. The epididymal fat pads were incubated separately for two hours in 2 ml of Krebs-Ringer bicarbonate buffer (KRB) containing 20 mMoles of glucose and 1 mg /ml of gelatin, with or without: (a) insulin: 50 µU/ml.; (b) ovine β-LPH: 0.1, 1.0 and 100 µg/50 mg of tissue wet weight. The incorporation of glucose carbons into CO2 and fatty acids was measured. The results indicate that at a concentration of 1.0 Mg/50 mg of wet tissue, ovine β-LPH stimulates the oxidation of glucose and its incorporation into fatty acids by rat epididymal fat. The observed effect could be due to: (1) a direct action of β-LPH on glucose utilization: "insulin-like activity"; (2) an indirect effect mediated through potentiation of endogenous (and exogenous) insulin; (3) enhanced re-esterification of free fatty acids released by lipolysis.

Key words

β-Lipotropic Hormone - Insulin - Lipogenesis - Lipolysis