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Horm Metab Res 1971; 3(1): 37-43
DOI: 10.1055/s-0028-1095045
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© Georg Thieme Verlag KG Stuttgart · New York

Preparation of B Cell Deficient Guinea Pig Islets of Langerhans

S. L. Howell , J. C. Edwards , M.  Whitfield
  • School of Biological Sciences, University of Sussex, Falmer, Brighton/Sussex, England
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Publication History

Publication Date:
07 January 2009 (online)

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Abstract

Islets of Langerhans were isolated from guinea pig pancreas six days after the animals had received an intraperitoneal injection of streptozotocin. These islets were depleted of at least 90% of their insulin content and of B cells as judged by immunoassay and by electron microscopy, while the ultrastructure of the other cell type remained entirely normal The secretory response of the A cells, which constituted about 70% of the cell population of the B cell deficient islets, were very similar to those of A cells in islets of Langerhans isolated from normal guinea pig. This preparation may provide the basis for a method of separating a population consisting solely of the glucagon-producing cell type.

Key words

Isolated Pancreatic Islets - Insulin Content - Streptozotocin - B-Cells - A-Cells

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