Hinterhandlähmungen bei Mastschweinen im Zusammenhang mit einem neuen Stamm des porzinen Teschovirus A11

Zusammenfassung

In einem Schweinemastbetrieb in Süddeutschland konnten über einen Zeitraum von 4 Wochen Hinterhandlähmungen bei 2 Altersgruppen (50 kg sowie 60 kg) beobachtet werden. Die Morbidität bei den beiden Altersgruppen war mit 3,3 % niedrig, der Großteil der betroffenen Tiere musste jedoch aufgrund einer fortschreitenden Paralyse der Hintergliedmaßen euthanasiert werden. Im Rahmen der pathomorphologischen Untersuchung von 2 Mastschweinen konnte eine hochgradige und diffuse lymphohistiozytäre Meningoenzephalomyelitis und Vaskulitis festgestellt werden. Immunhistochemisch wurde in allen Arealen des zentralen Nervensystems und in mehreren Zelltypen (Neuronen, Gliazellen, Endothelzellen, mononukleäre Zellen) Porzines Teschovirus-Antigen nachgewiesen. Die Untersuchung von Rückenmarksproben mittels PCR ergab den Nachweis von Porzinen Teschoviren. Der anschließend durchgeführten phylogenetischen Analyse zufolge wiesen die PCR positiven Proben die höchste Identität (88 % Vollgenomsequenz) zum Porzinen Teschovirus A11 Stamm „Dresden“ auf. Andere differentialdiagnostisch in Frage kommende Pathogene konnten mittels PCR, bakteriologischer Untersuchung und Sequenzierung nicht nachgewiesen werden. In dem Mastbestand traten nach ca. 4 Wochen keine weiteren Fälle von Lähmungserscheinungen mehr auf.

Abstract

In a fattening farm in southern Germany, paralysis of the hind limbs was observed in 2 age groups (50 kg as well as 60 kg) during a 4 week period. Despite a low morbidity of 3.3 % the majority of the affected animals needed to be euthanized in consequence to the progression of their hind limb paralysis. During pathomorphological examinations of 2 affected fattening pigs severe lymphohistiocytic meningoencephalomyelitis and vasculitis were detected. Immunhistochemistry revealed the presence of Porcine Teschovirus antigen in all parts of the central nervous system as well as in several cell types (neurons, glia cells, endothelial cells, mononuclear cells). Porcine Teschovirus was detected by PCR in spinal cord samples. The subsequently performed phylogenetic analysis PCR revealed a close relation (88 % full genome sequence) to porcine Teschovirus A11 strain “Dresden”. Other swine relevant pathogens were excluded by PCR, bacteriologic examination and sequencing. Following a period of 4 weeks no additional cases of hind limb paralysis were observed in the fattening farm.

Schlüsselwörter

Ataxie - Paralyse - Meningoenzephalomyelitis - Immunhistochemie - Sequenzierung

Key words

Ataxia - Paralysis - Meningoencephalomyelitis - Immunohistochemistry - Sequencing

Publication History

Received: 02 August 2021

Accepted: 29 November 2021

Article published online:
02 March 2022

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